Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CEQ83_RS18685 Genome accession   NZ_CP023317
Coordinates   3637878..3638318 (+) Length   146 a.a.
NCBI ID   WP_033579940.1    Uniprot ID   A0A806UCC3
Organism   Priestia megaterium strain A     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3632878..3643318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEQ83_RS18665 (CEQ83_18805) - 3633282..3633953 (-) 672 WP_028414985.1 response regulator transcription factor -
  CEQ83_RS18670 (CEQ83_18810) - 3634279..3635097 (+) 819 WP_028414986.1 squalene/phytoene synthase family protein -
  CEQ83_RS18675 (CEQ83_18815) - 3635133..3637082 (-) 1950 WP_028414987.1 FUSC family protein -
  CEQ83_RS18680 (CEQ83_18820) - 3637239..3637499 (-) 261 WP_013058561.1 metal-sensitive transcriptional regulator -
  CEQ83_RS18685 (CEQ83_18825) nucA/comI 3637878..3638318 (+) 441 WP_033579940.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CEQ83_RS18690 (CEQ83_18830) comJ 3638354..3638761 (+) 408 WP_098113593.1 competence protein ComJ -
  CEQ83_RS18695 (CEQ83_18835) - 3638863..3639453 (-) 591 WP_155017471.1 CPBP family intramembrane glutamic endopeptidase -
  CEQ83_RS18700 (CEQ83_18840) helD 3639842..3642160 (-) 2319 WP_108674279.1 RNA polymerase recycling motor HelD -
  CEQ83_RS27675 - 3642404..3642529 (-) 126 WP_014458690.1 hypothetical protein -
  CEQ83_RS18705 (CEQ83_18845) - 3642670..3643185 (-) 516 WP_108674280.1 enoyl-CoA hydratase/isomerase family protein -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16032.88 Da        Isoelectric Point: 4.4411

>NTDB_id=245979 CEQ83_RS18685 WP_033579940.1 3637878..3638318(+) (nucA/comI) [Priestia megaterium strain A]
MSTYGKKLMASLFIIIACVLGFIGHAAFLAPEKTEAEVNYDATLYFPLDRYPETGDHIRDAIAAGHSSVCTIDRDGAEAN
REESLKGYPTKTGYDRDEWPMAMCEEGGAGADIRYISPSDNRGAGSWVGNQLEQYPDGTRVQFIVQ

Nucleotide


Download         Length: 441 bp        

>NTDB_id=245979 CEQ83_RS18685 WP_033579940.1 3637878..3638318(+) (nucA/comI) [Priestia megaterium strain A]
ATGAGTACATACGGGAAAAAATTAATGGCAAGTCTATTCATTATCATTGCATGCGTTTTAGGGTTTATAGGACATGCGGC
GTTTTTAGCTCCTGAAAAAACGGAAGCCGAAGTAAACTATGATGCAACGCTTTATTTTCCACTGGACCGATATCCTGAAA
CGGGTGATCATATTCGCGACGCGATTGCCGCTGGTCACTCTTCCGTTTGCACAATTGATCGCGACGGTGCTGAAGCGAAT
CGGGAAGAATCGCTGAAAGGATACCCAACAAAAACAGGATATGACCGCGACGAATGGCCAATGGCTATGTGCGAAGAAGG
CGGAGCGGGAGCTGATATTCGCTATATTTCACCTTCTGATAACCGCGGAGCGGGCTCATGGGTTGGAAATCAATTAGAAC
AATACCCAGATGGTACACGCGTACAATTTATCGTGCAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806UCC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

65.079

86.301

0.562

Multiple sequence alignment