Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   B8B76_RS27090 Genome accession   NZ_CP023316
Coordinates   5663042..5664634 (+) Length   530 a.a.
NCBI ID   WP_021264116.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PPF-1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5658042..5669634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B8B76_RS27070 (B8B76_27025) pgeF 5659792..5660520 (-) 729 WP_015503650.1 peptidoglycan editing factor PgeF -
  B8B76_RS27075 (B8B76_27030) rluD 5660517..5661479 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B8B76_RS27080 (B8B76_27035) - 5661625..5662650 (+) 1026 WP_021264117.1 outer membrane protein assembly factor BamD -
  B8B76_RS27085 (B8B76_27040) - 5662807..5663052 (+) 246 WP_003102591.1 PP0621 family protein -
  B8B76_RS27090 (B8B76_27045) pilS 5663042..5664634 (+) 1593 WP_021264116.1 two-component system sensor histidine kinase PilS Regulator
  B8B76_RS27095 (B8B76_27050) pilR 5664649..5665986 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  B8B76_RS27100 (B8B76_27055) thiO 5666032..5667126 (-) 1095 WP_009316267.1 glycine oxidase ThiO -
  B8B76_RS27105 (B8B76_27060) - 5667261..5667770 (+) 510 WP_015503652.1 GspH/FimT family pseudopilin -
  B8B76_RS27110 (B8B76_27065) - 5667876..5668382 (+) 507 WP_003094696.1 GspH/FimT family pseudopilin -
  B8B76_RS27115 (B8B76_27070) pilV 5668373..5668930 (+) 558 WP_003094697.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59000.17 Da        Isoelectric Point: 6.8189

>NTDB_id=245936 B8B76_RS27090 WP_021264116.1 5663042..5664634(+) (pilS) [Pseudomonas aeruginosa strain PPF-1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SQLHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLDVIDNGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=245936 B8B76_RS27090 WP_021264116.1 5663042..5664634(+) (pilS) [Pseudomonas aeruginosa strain PPF-1]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGTAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
GCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGTCAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGATGTCATCGACAACGGTCCCGGCGTACCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.057

100

0.991

Multiple sequence alignment