Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CLI64_RS01985 Genome accession   NZ_CP023278
Coordinates   469344..470858 (-) Length   504 a.a.
NCBI ID   WP_103135658.1    Uniprot ID   A0A2I8A1N0
Organism   Nostoc sp. CENA543     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 464344..475858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI64_RS01940 (CLI64_01930) - 464427..464831 (+) 405 WP_103135650.1 deaminase -
  CLI64_RS01945 (CLI64_01935) - 464831..465340 (+) 510 WP_103135651.1 Imm1 family immunity protein -
  CLI64_RS01950 (CLI64_01940) - 465524..465730 (+) 207 WP_103135652.1 hypothetical protein -
  CLI64_RS01955 (CLI64_01945) - 465730..466080 (+) 351 WP_103135653.1 type II toxin-antitoxin system PemK/MazF family toxin -
  CLI64_RS01960 (CLI64_01950) - 466132..466656 (+) 525 WP_103135654.1 DUF433 domain-containing protein -
  CLI64_RS01965 (CLI64_01955) - 466650..467123 (+) 474 WP_103135655.1 hypothetical protein -
  CLI64_RS01970 (CLI64_01960) - 467260..467478 (+) 219 WP_103135656.1 UPF0175 family protein -
  CLI64_RS01975 (CLI64_01965) - 467733..468008 (-) 276 WP_192881662.1 hypothetical protein -
  CLI64_RS01980 (CLI64_01970) - 468426..469139 (-) 714 WP_103135657.1 Uma2 family endonuclease -
  CLI64_RS01985 (CLI64_01975) radA 469344..470858 (-) 1515 WP_103135658.1 DNA repair protein RadA Machinery gene
  CLI64_RS01990 (CLI64_01980) rpaB 471036..471764 (+) 729 WP_103135659.1 response regulator transcription factor RpaB -
  CLI64_RS02000 (CLI64_01985) - 471920..472561 (+) 642 WP_103135660.1 cofactor assembly of complex C subunit B -
  CLI64_RS02005 (CLI64_01990) - 472583..473212 (+) 630 WP_103135661.1 Uma2 family endonuclease -
  CLI64_RS02010 (CLI64_01995) - 473340..473846 (+) 507 WP_103135662.1 hypothetical protein -
  CLI64_RS02015 (CLI64_02000) - 473852..474130 (+) 279 WP_103135663.1 hypothetical protein -
  CLI64_RS02020 (CLI64_02005) - 474525..475049 (+) 525 WP_103135664.1 DUF456 domain-containing protein -

Sequence

Protein


Download         Length: 504 a.a.        Molecular weight: 54329.04 Da        Isoelectric Point: 4.9450

>NTDB_id=245704 CLI64_RS01985 WP_103135658.1 469344..470858(-) (radA) [Nostoc sp. CENA543]
MPKPKTIYICNECGAESPQWFGKCPNCGTYNSLEEQISIQSSVDIPSRGVSGWHSGQGNGKPANKPAKPRASLTFDQISD
RQVTRWESGYGELDRVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSNQLAQRYRILYVSGEESGQQVKLRASRLGVSKPLN
VVSDENDQEEEKEKESSPSIGADLYVLPETDLEEILREVDSLKPNVAVIDSIQTVFFPALTSAPGSVAQVRECTAALMKV
AKHEDITMLIVGHVTKEGAIAGPKVLEHLVDTVLYFEGDRFASHRLLRTVKNRFGATHEIGIFEMVENGLREVPNPSELF
LGNRDDPAPGTAIVVACEGTRPIVVELQALVSPTSYPSPRRAGTGIDYNRLVQILAVLEKRVGIPMSKLDSYVASAGGLN
VGEPAVDLGIAIAIVASFRDRIVDPGTVLIGEVGLGGQVRAVSQMELRLKEAAKLGFKRAIVPKGQKFPNFDIEIVPVSK
VIDAIIAAIPHQELTAEDLDIDEE

Nucleotide


Download         Length: 1515 bp        

>NTDB_id=245704 CLI64_RS01985 WP_103135658.1 469344..470858(-) (radA) [Nostoc sp. CENA543]
ATGCCAAAGCCAAAAACTATTTATATATGTAACGAATGCGGTGCAGAGTCTCCCCAGTGGTTTGGTAAGTGTCCTAATTG
TGGTACTTACAATTCCCTAGAAGAACAGATTAGCATCCAATCTTCTGTAGATATACCGAGTCGGGGGGTAAGCGGTTGGC
ATTCCGGCCAGGGTAACGGGAAGCCTGCGAATAAACCAGCTAAACCCAGAGCTTCTTTAACATTTGACCAAATCAGCGAT
CGCCAAGTCACCCGTTGGGAATCTGGATATGGTGAGTTAGATCGGGTGTTGGGTGGGGGAGTTGTTCCTGGTTCAATGGT
GTTAATTGGCGGTGATCCGGGTATCGGTAAATCTACCCTACTTTTGCAAGTATCAAATCAGCTAGCGCAGAGATACCGCA
TCCTGTACGTTTCGGGTGAAGAATCAGGACAACAAGTTAAATTAAGGGCTTCGCGTTTAGGTGTGTCTAAACCCTTAAAT
GTGGTAAGTGATGAAAATGACCAAGAAGAAGAAAAAGAAAAAGAATCATCACCAAGCATAGGTGCAGATTTATACGTCCT
GCCAGAAACCGATTTAGAAGAGATTTTACGGGAAGTAGATTCCTTAAAACCAAATGTGGCGGTAATTGACAGTATCCAAA
CCGTATTTTTCCCCGCTTTAACTTCCGCACCTGGTTCAGTAGCGCAAGTCAGGGAATGTACGGCTGCTTTGATGAAGGTG
GCAAAACATGAAGATATTACCATGTTAATTGTCGGACACGTAACTAAAGAAGGGGCGATCGCGGGACCGAAAGTATTAGA
ACATTTAGTAGATACGGTACTGTATTTTGAAGGCGATCGCTTTGCTTCCCATCGTTTGTTACGGACAGTCAAAAACCGCT
TCGGTGCAACCCACGAAATCGGGATTTTTGAGATGGTGGAAAACGGACTCAGAGAAGTCCCCAACCCCAGCGAGTTATTT
TTAGGCAACCGTGACGACCCCGCACCAGGCACAGCCATTGTAGTAGCTTGTGAAGGTACACGCCCCATCGTAGTTGAGTT
ACAAGCCTTAGTTAGTCCCACCAGTTACCCCTCACCCCGTCGGGCTGGAACGGGTATAGATTATAACCGCCTAGTGCAGA
TTCTCGCCGTCTTAGAAAAGCGCGTCGGGATACCCATGTCCAAATTAGATTCCTACGTAGCTTCGGCTGGAGGGTTAAAT
GTGGGAGAACCAGCCGTCGATTTAGGAATTGCTATTGCCATAGTTGCGAGTTTCCGCGATCGCATAGTAGATCCAGGTAC
AGTATTAATCGGTGAAGTCGGCTTGGGTGGACAAGTGCGCGCCGTTTCCCAGATGGAACTGAGGTTAAAAGAAGCCGCTA
AACTAGGATTTAAACGTGCGATCGTTCCGAAAGGTCAAAAATTCCCCAACTTTGACATTGAAATTGTACCAGTCTCCAAA
GTCATAGATGCCATCATCGCCGCCATCCCCCATCAAGAATTGACAGCCGAAGACTTAGACATAGACGAAGAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

KaiC (ATPase)

(89-293)

Subunit ChlI of Mg-chelatase (ChlI)

(404-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I8A1N0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.71

97.817

0.496
  radA Streptococcus pneumoniae Rx1

46.217

97.024

0.448
  radA Streptococcus pneumoniae D39

46.217

97.024

0.448
  radA Streptococcus pneumoniae R6

46.217

97.024

0.448
  radA Streptococcus pneumoniae TIGR4

46.217

97.024

0.448
  radA Streptococcus mitis SK321

46.107

96.825

0.446
  radA Streptococcus mitis NCTC 12261

47.845

92.063

0.44

Multiple sequence alignment