Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AOC34_RS09405 Genome accession   NZ_CP023276
Coordinates   1851161..1852048 (+) Length   295 a.a.
NCBI ID   WP_108469809.1    Uniprot ID   -
Organism   Polynucleobacter difficilis strain AM-8B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1846161..1857048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOC34_RS09375 pth 1846345..1846929 (+) 585 WP_108469805.1 aminoacyl-tRNA hydrolase -
  AOC34_RS09380 - 1846947..1847204 (-) 258 WP_108469806.1 YfhL family 4Fe-4S dicluster ferredoxin -
  AOC34_RS09385 coaD 1847209..1847706 (-) 498 WP_108469807.1 pantetheine-phosphate adenylyltransferase -
  AOC34_RS09390 rsmD 1847744..1848343 (-) 600 WP_234408095.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  AOC34_RS09395 - 1848347..1849708 (-) 1362 WP_234408096.1 pitrilysin family protein -
  AOC34_RS09400 - 1849705..1851078 (-) 1374 WP_108470156.1 pitrilysin family protein -
  AOC34_RS09405 pilA 1851161..1852048 (+) 888 WP_108469809.1 signal recognition particle-docking protein FtsY Machinery gene
  AOC34_RS09410 rpoH 1852206..1853078 (+) 873 WP_108469810.1 RNA polymerase sigma factor RpoH -
  AOC34_RS09415 - 1853092..1853718 (-) 627 WP_108469811.1 SCO family protein -
  AOC34_RS09420 cyoE 1853711..1854610 (-) 900 WP_108469812.1 heme o synthase -
  AOC34_RS09425 - 1854607..1855728 (-) 1122 WP_108469813.1 heme A synthase -
  AOC34_RS09430 - 1855725..1856384 (-) 660 WP_108469814.1 hypothetical protein -

Sequence

Protein


Download         Length: 295 a.a.        Molecular weight: 31441.08 Da        Isoelectric Point: 7.1200

>NTDB_id=245702 AOC34_RS09405 WP_108469809.1 1851161..1852048(+) (pilA) [Polynucleobacter difficilis strain AM-8B5]
MFGLRKTLGSLFKSSRTDEAWFDTLEESLIQSDVGLPTTERLMETLRAAAKSEKAASPEALQALLIDAVSALLSTLEANP
NPLFMQSAHRPEVWLVVGVNGAGKTTTIGKLCHHFQSQERAVLLAAGDTFRAAARNQLKEWAVRNGVDLVSQETGDAAAV
AHDAIHAAIARKSDVLIIDTAGRLATQDNLMEELKKVKRVISKALPGAPHQILLVLDGNTGQNGISQVKAFHAALGLTGL
IVTKLDGTAKGGVICALAQTFQGEAKPLIFALGKGEGIQDLVPFNAKQYSTELFN

Nucleotide


Download         Length: 888 bp        

>NTDB_id=245702 AOC34_RS09405 WP_108469809.1 1851161..1852048(+) (pilA) [Polynucleobacter difficilis strain AM-8B5]
ATGTTTGGATTACGTAAAACCCTAGGGTCCCTGTTTAAATCAAGCCGCACGGATGAGGCATGGTTTGACACACTGGAAGA
ATCACTGATTCAAAGTGATGTTGGCTTACCCACTACGGAGCGGCTGATGGAGACCCTGCGTGCCGCCGCGAAGTCAGAAA
AAGCTGCTTCCCCAGAAGCCCTGCAAGCACTCCTAATTGATGCCGTTAGCGCCCTACTCTCGACTCTTGAGGCCAATCCC
AATCCCCTATTTATGCAATCGGCGCATCGACCCGAGGTATGGCTCGTGGTTGGCGTAAATGGCGCCGGCAAAACGACGAC
AATCGGCAAGCTCTGCCACCACTTTCAGTCGCAAGAACGTGCCGTTCTATTGGCGGCAGGCGATACCTTTCGAGCGGCAG
CGCGCAATCAACTGAAAGAGTGGGCGGTGCGTAATGGTGTAGACCTGGTTAGTCAGGAAACCGGTGATGCTGCTGCTGTG
GCACATGACGCCATCCACGCTGCCATTGCGCGAAAAAGTGATGTCTTAATTATTGATACGGCTGGCCGCCTAGCCACTCA
AGATAACTTGATGGAAGAGCTTAAAAAGGTCAAGCGCGTGATTAGCAAGGCCTTGCCAGGAGCGCCCCATCAAATCCTCT
TAGTCCTGGATGGCAATACTGGTCAAAACGGCATTAGTCAGGTAAAGGCTTTCCATGCCGCCCTAGGCCTCACAGGATTA
ATTGTTACGAAGCTGGATGGCACGGCCAAAGGCGGGGTGATTTGTGCCTTGGCGCAGACCTTCCAGGGGGAGGCTAAGCC
CCTCATCTTTGCGCTGGGCAAAGGAGAGGGTATTCAGGACCTGGTGCCCTTCAATGCAAAGCAGTATTCGACCGAATTAT
TCAACTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(91-294)

SRP54-type protein, helical bundle domain (SRP54_N)

(4-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.171

99.322

0.498

Multiple sequence alignment