Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CLM73_RS00665 Genome accession   NZ_CP023270
Coordinates   136828..138279 (+) Length   483 a.a.
NCBI ID   WP_105236895.1    Uniprot ID   A0A2S0I155
Organism   Achromobacter spanius strain MYb73     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131828..143279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLM73_RS00650 (CLM73_00650) - 132581..133909 (+) 1329 WP_105236892.1 amidase -
  CLM73_RS00655 (CLM73_00655) leuA 134040..135749 (-) 1710 WP_105236893.1 2-isopropylmalate synthase -
  CLM73_RS00660 (CLM73_00660) - 136191..136829 (-) 639 WP_105236894.1 hypothetical protein -
  CLM73_RS00665 (CLM73_00665) rpoN/rpoN1 136828..138279 (+) 1452 WP_105236895.1 RNA polymerase factor sigma-54 Machinery gene
  CLM73_RS00670 (CLM73_00670) - 138401..138679 (+) 279 WP_105236896.1 (2Fe-2S)-binding protein -
  CLM73_RS00680 (CLM73_00680) - 138974..139885 (+) 912 WP_105236897.1 DMT family transporter -
  CLM73_RS00685 (CLM73_00685) bfr 140262..140738 (+) 477 WP_056327755.1 bacterioferritin -
  CLM73_RS00690 (CLM73_00690) - 140839..141513 (-) 675 WP_105236898.1 hypothetical protein -
  CLM73_RS00695 (CLM73_00695) - 141551..143158 (-) 1608 WP_056565700.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 483 a.a.        Molecular weight: 52910.22 Da        Isoelectric Point: 4.8739

>NTDB_id=245631 CLM73_RS00665 WP_105236895.1 136828..138279(+) (rpoN/rpoN1) [Achromobacter spanius strain MYb73]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPSSAEAESRSESERESSVQDD
EPAAERETPIDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDVALASLLIDELDENGYLGSPLDEILGW
LPAEMEPDMDELRAALSLLQSFDPAGIGARDMADCLHLQLRNPDLTRLPEAADPAVLACARRICAEHLPLLATGNAVRLC
AALGCDEPTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQVNGLYAQMLSNQKESAHA
GLQAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTL
ELKRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQA
AAR

Nucleotide


Download         Length: 1452 bp        

>NTDB_id=245631 CLM73_RS00665 WP_105236895.1 136828..138279(+) (rpoN/rpoN1) [Achromobacter spanius strain MYb73]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
GCAATCGATCCGGTTGCTGCAGCTGTCGACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAAAATCCCCTGC
TGGATCGGGAGGACGAGCCGTCGTCGGCCGAGGCGGAGTCGCGGTCCGAGTCCGAGCGCGAATCCTCCGTGCAGGACGAC
GAGCCGGCGGCCGAGCGCGAGACGCCCATCGACGAAATGCCGGGTTCGGGCGGCGTGTACCCCGATGACGACGCCAGCCT
GCCCGAGGCGGCGCGTCCGGACACCTTGCGCGAGCACCTGCTGGGCCAGCTTGCCTTGACCCGGGCGGCCCCGCGCGATG
TCGCGCTGGCGTCCCTGTTGATCGACGAACTGGACGAAAACGGCTATCTCGGATCGCCGCTGGACGAAATCCTGGGCTGG
CTCCCTGCCGAAATGGAGCCCGACATGGACGAGCTGCGGGCGGCGTTGTCGCTGCTGCAGTCGTTTGATCCCGCCGGCAT
TGGCGCGCGCGACATGGCTGACTGCTTGCACCTTCAGTTGCGCAATCCCGACCTCACCCGGTTGCCCGAGGCCGCTGACC
CCGCCGTGCTGGCCTGTGCCCGGCGTATCTGCGCCGAGCACCTGCCCCTGCTCGCGACCGGCAACGCCGTGCGCCTTTGT
GCGGCGCTCGGCTGCGACGAGCCCACGTTTCGCGCGGCGCATTCGCTGATCCTGCGCCTGGAGCCCCGGCCCGGGCGGGC
ATGGACGGTGCCGGCGGCCGAGTACGCGGTGCCGGACGTCATCGTGCGCAAAACCCGGCGCGGCTGGCAGGCCACGCTCA
ACAGCGCCGCGGTGCCGCGGCTGCAAGTCAACGGCCTGTACGCGCAGATGCTCAGCAATCAGAAGGAGTCCGCGCACGCC
GGGCTGCAGGCCCAACTGCAGCAGGCCAAATGGATGATCCGGAATGTGGAGCAGCGTTTTGACACCATCCTGCGTGTGTC
GCAGGCCATCGTCGAACGCCAGACGGCATTCTTCAGCCAGGGCCCGGCGGCCATGCGGCCGCTCATCCTGAAAGACATCG
CCGGCGAACTGGGATTGCACGAGTCGACCATTTCACGCGCCACAACACAGAAGTACATGCTCACTCCGTTTGGCACGCTG
GAGCTTAAGCGCTTCTTTGGCACGGGGGTTTCGACCGAAGGCGGCGACGCCACGTCGGCAACGGCGGTGCAGACCTACAT
CCGCCAGATGGTGGCCGACGAAAATCGCGCCAAGCCGTTGTCGGACAGCCAGATCATGCAAAAACTGGCCGATCAGGGGA
TAGTGATCGCCCGTCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCGGCCGCGCTGCGCAAGGCGCAAGCC
GCCGCCCGCTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(110-307)

Sigma-54, DNA binding domain (Sigma54_DBD)

(322-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.901

100

0.52

Multiple sequence alignment