Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CLD04_RS13535 Genome accession   NZ_CP023257
Coordinates   2523754..2524164 (-) Length   136 a.a.
NCBI ID   WP_095843847.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2518754..2529164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS13505 (CLD04_13505) - 2518937..2519077 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  CLD04_RS13510 (CLD04_13510) yqeF 2519336..2520067 (-) 732 WP_095843845.1 SGNH/GDSL hydrolase family protein -
  CLD04_RS13515 (CLD04_13515) cwlH 2520319..2521033 (-) 715 Protein_2628 N-acetylmuramoyl-L-alanine amidase -
  CLD04_RS13520 (CLD04_13520) yqeD 2521220..2521846 (+) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  CLD04_RS13525 (CLD04_13525) gnd 2521865..2522758 (-) 894 WP_041333751.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  CLD04_RS13530 (CLD04_13530) yqeB 2522999..2523721 (+) 723 WP_095843846.1 hypothetical protein -
  CLD04_RS13535 (CLD04_13535) nucA/comI 2523754..2524164 (-) 411 WP_095843847.1 sporulation-specific Dnase NucB Machinery gene
  CLD04_RS13540 (CLD04_13540) sigK 2524360..2525088 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  CLD04_RS13545 (CLD04_13545) - 2525660..2526340 (-) 681 WP_015714344.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
  CLD04_RS13550 (CLD04_13550) - 2526471..2527813 (+) 1343 Protein_2635 MATE family efflux transporter -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 15000.03 Da        Isoelectric Point: 5.1853

>NTDB_id=245561 CLD04_RS13535 WP_095843847.1 2523754..2524164(-) (nucA/comI) [Bacillus subtilis strain TLO3]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKMLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=245561 CLD04_RS13535 WP_095843847.1 2523754..2524164(-) (nucA/comI) [Bacillus subtilis strain TLO3]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAATGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment