Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CLD04_RS13290 Genome accession   NZ_CP023257
Coordinates   2480411..2481178 (-) Length   255 a.a.
NCBI ID   WP_017696274.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2475411..2486178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS13270 (CLD04_13270) ppsR 2475586..2476398 (-) 813 WP_003230064.1 pyruvate, water dikinase regulatory protein -
  CLD04_RS13275 (CLD04_13275) ccpN 2476424..2477062 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  CLD04_RS13280 (CLD04_13280) glyS 2477195..2479234 (-) 2040 WP_014480291.1 glycine--tRNA ligase subunit beta -
  CLD04_RS13285 (CLD04_13285) glyQ 2479227..2480114 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  CLD04_RS13290 (CLD04_13290) recO 2480411..2481178 (-) 768 WP_017696274.1 DNA repair protein RecO Machinery gene
  CLD04_RS13295 (CLD04_13295) yqzL 2481215..2481358 (-) 144 WP_003230053.1 YqzL family protein -
  CLD04_RS13300 (CLD04_13300) era 2481506..2482411 (-) 906 WP_003230051.1 GTPase Era -
  CLD04_RS13305 (CLD04_13305) - 2482392..2482802 (-) 411 WP_014480293.1 cytidine deaminase -
  CLD04_RS13310 (CLD04_13310) unpK 2482921..2483292 (-) 372 WP_041351685.1 diacylglycerol kinase family protein -
  CLD04_RS13315 (CLD04_13315) ybeY 2483273..2483746 (-) 474 WP_041351686.1 rRNA maturation RNase YbeY -
  CLD04_RS13320 (CLD04_13320) pgpH 2483747..2485882 (-) 2136 WP_072557183.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29346.03 Da        Isoelectric Point: 8.3080

>NTDB_id=245557 CLD04_RS13290 WP_017696274.1 2480411..2481178(-) (recO) [Bacillus subtilis strain TLO3]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKTNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=245557 CLD04_RS13290 WP_017696274.1 2480411..2481178(-) (recO) [Bacillus subtilis strain TLO3]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGAC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATTAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.216

100

0.992

Multiple sequence alignment