Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CLD04_RS02360 Genome accession   NZ_CP023257
Coordinates   424452..424901 (-) Length   149 a.a.
NCBI ID   WP_124060108.1    Uniprot ID   -
Organism   Bacillus subtilis strain TLO3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 419452..429901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLD04_RS02330 (CLD04_02330) yckA 419727..420407 (-) 681 WP_017696121.1 amino acid ABC transporter permease -
  CLD04_RS02335 (CLD04_02335) yckB 420417..421280 (-) 864 WP_072557073.1 transporter substrate-binding domain-containing protein -
  CLD04_RS21540 - 421443..421633 (+) 191 Protein_417 hypothetical protein -
  CLD04_RS02340 (CLD04_02340) - 421678..421989 (+) 312 Protein_418 RDD family protein -
  CLD04_RS02345 (CLD04_02345) yckD 422071..422403 (+) 333 WP_015252849.1 YckD family protein -
  CLD04_RS02350 (CLD04_02350) bglC 422557..423990 (+) 1434 WP_095843470.1 6-phospho-beta-glucosidase -
  CLD04_RS02355 (CLD04_02355) nin/comJ 424027..424425 (-) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  CLD04_RS02360 (CLD04_02360) nucA/comI 424452..424901 (-) 450 WP_124060108.1 DNA-entry nuclease Machinery gene
  CLD04_RS02365 (CLD04_02365) tlpC 425069..426790 (-) 1722 WP_095843471.1 methyl-accepting chemotaxis protein TlpC -
  CLD04_RS02370 (CLD04_02370) hxlB 426901..427458 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  CLD04_RS02375 (CLD04_02375) hxlA 427464..428096 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  CLD04_RS02380 (CLD04_02380) hxlR 428325..428687 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16456.39 Da        Isoelectric Point: 4.5282

>NTDB_id=245516 CLD04_RS02360 WP_124060108.1 424452..424901(-) (nucA/comI) [Bacillus subtilis strain TLO3]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETISFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=245516 CLD04_RS02360 WP_124060108.1 424452..424901(-) (nucA/comI) [Bacillus subtilis strain TLO3]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCTCCTTCCCTTCTGACCGTT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98

Multiple sequence alignment