Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CJ306_RS20995 Genome accession   NZ_CP023245
Coordinates   4067739..4068173 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain HBL-AI     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4062739..4073173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ306_RS20980 (CJ306_21520) - 4063915..4064472 (+) 558 WP_119683878.1 PadR family transcriptional regulator -
  CJ306_RS20985 (CJ306_21525) metE 4064986..4067274 (+) 2289 WP_001007608.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CJ306_RS20990 (CJ306_21530) comJ 4067313..4067702 (-) 390 WP_000424030.1 competence protein ComJ -
  CJ306_RS20995 (CJ306_21535) nucA/comI 4067739..4068173 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  CJ306_RS21000 (CJ306_21545) - 4068400..4069149 (+) 750 WP_000388471.1 DUF3967 domain-containing protein -
  CJ306_RS21005 (CJ306_21550) kinB 4069188..4070462 (-) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  CJ306_RS21010 (CJ306_21555) - 4070772..4070972 (+) 201 WP_000929280.1 hypothetical protein -
  CJ306_RS21015 (CJ306_21560) - 4071095..4072258 (+) 1164 WP_098583044.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=245375 CJ306_RS20995 WP_000811494.1 4067739..4068173(-) (nucA/comI) [Bacillus cereus strain HBL-AI]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=245375 CJ306_RS20995 WP_000811494.1 4067739..4068173(-) (nucA/comI) [Bacillus cereus strain HBL-AI]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGTTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment