Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CLI14_RS01660 Genome accession   NZ_CP023208
Coordinates   360542..361552 (+) Length   336 a.a.
NCBI ID   WP_013855885.1    Uniprot ID   A0AAW4AGY3
Organism   Vibrio anguillarum strain ATCC-68554     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355542..366552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI14_RS01645 (CLI14_01645) - 356092..356820 (-) 729 WP_013855882.1 redoxin family protein -
  CLI14_RS01650 (CLI14_01650) oxyR 356967..357857 (+) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  CLI14_RS01655 (CLI14_01655) - 357894..360392 (-) 2499 WP_013855884.1 PBP1A family penicillin-binding protein -
  CLI14_RS01660 (CLI14_01660) pilM 360542..361552 (+) 1011 WP_013855885.1 type IV pilus assembly protein PilM Machinery gene
  CLI14_RS01665 (CLI14_01665) pilN 361542..362144 (+) 603 WP_013855886.1 PilN domain-containing protein Machinery gene
  CLI14_RS01670 (CLI14_01670) pilO 362137..362730 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  CLI14_RS01675 (CLI14_01675) pilP 362717..363238 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  CLI14_RS01680 (CLI14_01680) pilQ 363262..364977 (+) 1716 WP_013855889.1 type IV pilus secretin PilQ family protein Machinery gene
  CLI14_RS01685 (CLI14_01685) aroK 365164..365685 (+) 522 WP_010317691.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37439.00 Da        Isoelectric Point: 7.2859

>NTDB_id=245301 CLI14_RS01660 WP_013855885.1 360542..361552(+) (pilM) [Vibrio anguillarum strain ATCC-68554]
MGKSMVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSHKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRLRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=245301 CLI14_RS01660 WP_013855885.1 360542..361552(+) (pilM) [Vibrio anguillarum strain ATCC-68554]
ATGGGTAAATCAATGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCACAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAAATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCCGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGTAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGTGCAGATACTCCGATGTTGGCGCAAGAGATTTCGCGGCGTCTCAAGCTTGAGTGCGAAATCCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCTGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCGGCT
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.381

100

0.524

Multiple sequence alignment