Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CIW72_RS05865 Genome accession   NZ_CP023159
Coordinates   1264105..1265841 (+) Length   578 a.a.
NCBI ID   WP_005915821.1    Uniprot ID   -
Organism   Xanthomonas citri pv. malvacearum strain MS14003     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1259105..1270841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CIW72_RS05840 (CIW72_05840) - 1260373..1260789 (-) 417 WP_033481911.1 pilin -
  CIW72_RS23240 - 1261232..1261516 (+) 285 WP_232515052.1 SMR family transporter -
  CIW72_RS05850 (CIW72_05850) - 1261521..1262315 (+) 795 WP_005915828.1 glycosyltransferase -
  CIW72_RS05855 (CIW72_05855) - 1262318..1263358 (+) 1041 WP_050554799.1 glycosyltransferase -
  CIW72_RS05860 (CIW72_05860) - 1263351..1264007 (+) 657 WP_157733690.1 methionine biosynthesis protein MetW -
  CIW72_RS05865 (CIW72_05865) pilB 1264105..1265841 (+) 1737 WP_005915821.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CIW72_RS05870 (CIW72_05870) pilB 1265938..1266057 (+) 120 WP_005915819.1 hypothetical protein Machinery gene
  CIW72_RS05875 (CIW72_05875) - 1266213..1266356 (+) 144 WP_005915817.1 hypothetical protein -
  CIW72_RS05880 (CIW72_05880) pilR 1266543..1267997 (-) 1455 WP_033481907.1 sigma-54 dependent transcriptional regulator Regulator
  CIW72_RS05885 (CIW72_05885) - 1268262..1269875 (-) 1614 WP_005915814.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62606.69 Da        Isoelectric Point: 5.7896

>NTDB_id=245018 CIW72_RS05865 WP_005915821.1 1264105..1265841(+) (pilB) [Xanthomonas citri pv. malvacearum strain MS14003]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFIGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVKKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=245018 CIW72_RS05865 WP_005915821.1 1264105..1265841(+) (pilB) [Xanthomonas citri pv. malvacearum strain MS14003]
ATGAATAGCGTCGTCACAGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCAAAGGTCCCGTTGCCTCAATGGTTCTCGGAGAAAAAGTTGG
TGACAGCTTCGCAGCTGGCGGCCGCCAATGCAGTCGAGTTCGGCATGCCGCTGCTGGATGTATCGGCGTTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTCAGTGAGGAGCTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCATAGGGGTGAGCAACCCGACACAGACTCGCGCGCTGGACGACATCAAGTTCCATACGAACTTAGTGGTCGAGGCGA
TCCTCGTCGACGAAGATCAGATCCGCCGGACGCTCGAGCAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCTTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCGACCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAAAAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGACGGCTTGTTGAAGAACGTGGCGAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AAGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAG
CTGTTCCTGGAGGCGATCCACAAGCCGTACGGGATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGCATCCTCAACGACGAGACACGCAATATCTCCACCGCGGAGGACCCGGTTGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGAATCGCGCCATACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAAGCGGTCGGTTGCGA
TGAGTGCACCGAAGGTTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCAATCGTGC
TGGAAGGCGGGAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Legionella pneumophila strain ERS1305867

52.197

98.443

0.514
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

49.819

95.848

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.922

97.578

0.448
  pilB Vibrio campbellii strain DS40M4

44.87

99.481

0.446

Multiple sequence alignment