Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M28_RS01185 Genome accession   NC_007296
Coordinates   209530..210891 (+) Length   453 a.a.
NCBI ID   WP_002991192.1    Uniprot ID   Q1JNL0
Organism   Streptococcus pyogenes MGAS6180     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 176999..209449 209530..210891 flank 81


Gene organization within MGE regions


Location: 176999..210891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M28_RS01030 (M28_Spy0157) polA 176999..179641 (+) 2643 WP_011284469.1 DNA polymerase I -
  M28_RS01035 (M28_Spy0158) - 179828..180283 (+) 456 WP_002987917.1 CoA-binding protein -
  M28_RS01040 (M28_Spy0159) perR 180335..180802 (+) 468 WP_002987920.1 peroxide-responsive transcriptional repressor PerR -
  M28_RS01045 (M28_Spy0160) - 181010..181258 (+) 249 WP_228444955.1 hypothetical protein -
  M28_RS01050 (M28_Spy0161) - 181480..182814 (+) 1335 WP_002987923.1 phosphoadenosine phosphosulfate reductase -
  M28_RS01055 (M28_Spy0162) - 182807..183337 (+) 531 WP_002987925.1 IbrB-like domain-containing protein -
  M28_RS09715 - 183384..183533 (-) 150 Protein_159 ISL3 family transposase -
  M28_RS01065 - 183666..184816 (-) 1151 Protein_160 IS3 family transposase -
  M28_RS01070 (M28_Spy0167) - 184894..186204 (-) 1311 WP_011284473.1 SLC13 family permease -
  M28_RS01075 (M28_Spy0168) nadC 186428..187300 (+) 873 WP_021340668.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M28_RS01085 (M28_Spy0171) - 189028..189891 (-) 864 WP_002986324.1 DUF975 family protein -
  M28_RS10000 (M28_Spy0172) - 189928..190113 (+) 186 WP_002986321.1 hypothetical protein -
  M28_RS01095 (M28_Spy0173) tgt 190110..191252 (+) 1143 WP_002986319.1 tRNA guanosine(34) transglycosylase Tgt -
  M28_RS01100 (M28_Spy0174) - 191469..191780 (+) 312 WP_002987947.1 CHY zinc finger protein -
  M28_RS01105 (M28_Spy0175) - 191784..192320 (+) 537 WP_011284477.1 biotin transporter BioY -
  M28_RS01110 (M28_Spy0176) - 192463..193242 (+) 780 WP_002986311.1 MBL fold metallo-hydrolase -
  M28_RS01115 (M28_Spy0177) tadA 193242..193757 (+) 516 WP_002992549.1 tRNA adenosine(34) deaminase TadA -
  M28_RS01120 (M28_Spy0178) - 194371..195603 (-) 1233 WP_011284478.1 transglutaminase domain-containing protein -
  M28_RS01130 (M28_Spy0180) speG 196003..196707 (+) 705 WP_010921857.1 streptococcal pyrogenic exotoxin SpeG -
  M28_RS01135 (M28_Spy0183) - 197163..198512 (+) 1350 WP_011284480.1 glucose-6-phosphate isomerase -
  M28_RS01140 (M28_Spy0184) - 198861..200369 (-) 1509 WP_011284481.1 helix-turn-helix domain-containing protein -
  M28_RS01145 (M28_Spy0186) - 201057..201728 (+) 672 WP_011284482.1 rhomboid family intramembrane serine protease -
  M28_RS01150 (M28_Spy0187) galU 201827..202726 (-) 900 WP_002986125.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  M28_RS01155 (M28_Spy0188) - 202759..203775 (-) 1017 WP_011284483.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  M28_RS01160 (M28_Spy0189) - 204073..204522 (+) 450 WP_002986120.1 MarR family winged helix-turn-helix transcriptional regulator -
  M28_RS01165 (M28_Spy0190) - 204515..206221 (+) 1707 WP_011284484.1 ABC transporter ATP-binding protein -
  M28_RS01170 (M28_Spy0191) - 206224..208008 (+) 1785 WP_021340815.1 ABC transporter ATP-binding protein -
  M28_RS01175 (M28_Spy0192) - 208126..208893 (+) 768 WP_011054158.1 epoxyqueuosine reductase QueH -
  M28_RS01180 (M28_Spy0193) - 209003..209449 (+) 447 WP_002986111.1 dUTP diphosphatase -
  M28_RS01185 (M28_Spy0194) radA 209530..210891 (+) 1362 WP_002991192.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49358.62 Da        Isoelectric Point: 6.0962

>NTDB_id=24468 M28_RS01185 WP_002991192.1 209530..210891(+) (radA) [Streptococcus pyogenes MGAS6180]
MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRV
LGGGVVPGSLILIGGDPGIGKSTLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKAVFS

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=24468 M28_RS01185 WP_002991192.1 209530..210891(+) (radA) [Streptococcus pyogenes MGAS6180]
ATGGCAAAGAAAAAAGCAACCTTTATCTGTCAGGAGTGTGGCTACCAGTCGCCAAAGTATTTAGGGCGCTGTCCAAATTG
CTCGGCCTGGTCTTCTTTTGTGGAAGAAGTAGAGGTCAAAGAAGTCAAAAATGCCCGTGTTAGTCTAGCTGGAGAAAAGT
CCAGACCGGTTAAACTAAAAGATGTGGATAATATTAGTTATCACAGAACACAGACTGACATGTCTGAATTTAATAGAGTT
CTTGGAGGGGGTGTGGTTCCTGGAAGCTTGATTTTAATTGGTGGTGATCCAGGTATTGGAAAATCCACCTTACTCTTGCA
GGTATCTACGCAGCTCGCTAATAAGGGAACGGTTCTCTATGCGTCTGGGGAAGAATCAGCAGAGCAGATTAAACTGCGGA
GTGAGCGTCTGGGTGATATTGACAATGAATTTTACCTCTATGCCGAAACCAATATGCAAGCTATTCGCACAGAAATTGAA
AATATTAAACCAGATTTTTTAATTATTGACTCCATTCAGACCATCATGAGCCCTGATATTACAGGTGTTCAAGGATCAGT
CAGTCAGGTGAGAGAAGTCACTGCAGAATTGATGCAGCTTGCAAAGACGAACAACATTGCAACCTTTATTGTGGGTCATG
TCACCAAGGAAGGAACCTTGGCTGGGCCTCGTATGTTAGAACACATGGTGGATACCGTTCTCTACTTTGAAGGGGAAAGG
CATCACACCTTTCGCATTCTTCGGGCTGTGAAAAACCGCTTTGGCTCCACTAACGAGATTGGCATCTTTGAAATGCAGTC
TGGCGGCTTGGTAGAGGTCTTAAATCCTAGTCAAGTCTTTTTAGAGGAACGCTTAGATGGGGCAACAGGATCGGCTGTCG
TTGTAACGATGGAAGGAAGTCGTCCTATCTTAGCAGAGGTGCAATCTTTAGTGACACCAACCGTTTTTGGCAATGCCAGA
CGAACCACTACGGGACTTGATTTCAATCGCGTAAGCCTAATAATGGCAGTTTTAGAAAAGCGTTGTGGTTTACTCTTACA
AAATCAAGATGCCTACCTCAAGTCGGCTGGTGGTGTCAAACTAGACGAACCTGCCATTGATTTGGCAGTCGCTGTGGCTA
TCGCATCCAGTTATAAGGAAAAACCAACCAGCCCTCAAGAAGCTTTCTTAGGCGAGATCGGGTTAACAGGTGAGATTCGG
CGAGTGACCCGTATTGAGCAACGGATTAATGAAGCAGCAAAGCTTGGCTTTACCAAGGTCTATGCTCCTAAAAATGCCTT
GCAAGGCATTGATATCCCTCAAGGTATTGAGGTTGTTGGAGTGACTACGGTTGGTCAGGTTTTAAAGGCAGTTTTTAGCT
AG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(56-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1JNL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

85.651

100

0.857
  radA Streptococcus pneumoniae D39

85.43

100

0.854
  radA Streptococcus pneumoniae R6

85.43

100

0.854
  radA Streptococcus pneumoniae Rx1

85.43

100

0.854
  radA Streptococcus mitis SK321

85.43

100

0.854
  radA Streptococcus pneumoniae TIGR4

85.43

100

0.854
  radA Bacillus subtilis subsp. subtilis str. 168

61.369

100

0.614

Multiple sequence alignment