Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CJF63_RS10305 Genome accession   NZ_CP023001
Coordinates   1826140..1827183 (-) Length   347 a.a.
NCBI ID   WP_001013201.1    Uniprot ID   A0A6L8PIF1
Organism   Bacillus anthracis strain 14RA5914     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1821140..1832183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJF63_RS10260 - 1821568..1821768 (-) 201 WP_000106085.1 YqzE family protein -
  CJF63_RS10265 aroK 1821807..1822304 (-) 498 WP_000836640.1 shikimate kinase AroK -
  CJF63_RS10270 - 1822425..1823075 (-) 651 WP_000183878.1 2OG-Fe(II) oxygenase -
  CJF63_RS10275 comGG 1823251..1823621 (-) 371 Protein_1934 competence type IV pilus minor pilin ComGG -
  CJF63_RS10280 comGF 1823618..1824088 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  CJF63_RS10285 comGE 1824058..1824360 (-) 303 WP_000829456.1 competence type IV pilus minor pilin ComGE -
  CJF63_RS10290 comGD 1824353..1824808 (-) 456 WP_000810395.1 comG operon protein ComGD -
  CJF63_RS10295 comGC 1824805..1825104 (-) 300 WP_001178688.1 comG operon protein ComGC -
  CJF63_RS10300 comGB 1825116..1826147 (-) 1032 WP_000471910.1 comG operon protein ComGB -
  CJF63_RS10305 comGA 1826140..1827183 (-) 1044 WP_001013201.1 competence protein ComGA Machinery gene
  CJF63_RS10310 - 1827389..1828084 (+) 696 WP_000434119.1 helix-turn-helix transcriptional regulator -
  CJF63_RS10315 - 1828210..1828452 (+) 243 WP_000440708.1 DUF2626 domain-containing protein -
  CJF63_RS10320 - 1828560..1829954 (+) 1395 WP_001094329.1 L-cystine transporter -
  CJF63_RS10325 - 1830129..1830536 (-) 408 WP_000877909.1 hypothetical protein -
  CJF63_RS10330 - 1830624..1830839 (-) 216 WP_001008320.1 DUF3912 family protein -
  CJF63_RS10335 - 1831092..1831403 (+) 312 WP_001093245.1 hypothetical protein -
  CJF63_RS10340 - 1831440..1831928 (-) 489 WP_000764252.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39354.93 Da        Isoelectric Point: 9.3530

>NTDB_id=243950 CJF63_RS10305 WP_001013201.1 1826140..1827183(-) (comGA) [Bacillus anthracis strain 14RA5914]
MNGIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMGFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=243950 CJF63_RS10305 WP_001013201.1 1826140..1827183(-) (comGA) [Bacillus anthracis strain 14RA5914]
ATGAATGGAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGTCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGGTTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6L8PIF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment