Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CJ010_RS12000 Genome accession   NZ_CP022958
Coordinates   2585834..2587207 (+) Length   457 a.a.
NCBI ID   WP_141018244.1    Uniprot ID   A0A4Y6KT53
Organism   Azoarcus sp. DD4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2580834..2592207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJ010_RS11970 - 2581114..2581401 (+) 288 WP_141018239.1 hypothetical protein -
  CJ010_RS11975 (CJ010_11915) tsaB 2581458..2582147 (+) 690 WP_141018240.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB -
  CJ010_RS11980 (CJ010_11920) rimI 2582177..2582599 (+) 423 WP_141020679.1 ribosomal protein S18-alanine N-acetyltransferase -
  CJ010_RS11985 (CJ010_11925) - 2582599..2583480 (+) 882 WP_141018241.1 uracil-DNA glycosylase family protein -
  CJ010_RS11990 (CJ010_11930) lplT 2583508..2584758 (-) 1251 WP_141018242.1 lysophospholipid transporter LplT -
  CJ010_RS11995 (CJ010_11935) - 2584914..2585834 (+) 921 WP_141018243.1 LysR family transcriptional regulator -
  CJ010_RS12000 (CJ010_11940) radA 2585834..2587207 (+) 1374 WP_141018244.1 DNA repair protein RadA Machinery gene
  CJ010_RS12005 (CJ010_11945) - 2587204..2588127 (-) 924 WP_141018245.1 LysR substrate-binding domain-containing protein -
  CJ010_RS12010 (CJ010_11950) - 2588265..2589644 (+) 1380 WP_141018246.1 L-serine ammonia-lyase -
  CJ010_RS12015 (CJ010_11955) sstT 2589735..2590970 (+) 1236 WP_141018247.1 serine/threonine transporter SstT -
  CJ010_RS12020 (CJ010_11960) - 2591001..2592026 (+) 1026 WP_141018248.1 low specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 48445.61 Da        Isoelectric Point: 7.1658

>NTDB_id=243717 CJ010_RS12000 WP_141018244.1 2585834..2587207(+) (radA) [Azoarcus sp. DD4]
MAKARTVFVCTECGGQALRWQGQCPQCSAWNTLVESVAEQGGASGRFAALAGNTSRLQALSELQPREEPRTPTGIDEFDR
VLGGGLVAGGVVLIGGDPGIGKSTLLLQALSHLAADQAAVYVSGEESGEQVALRAQRLQLAPTGLQLLAEINLERILASL
RDARPRVAVIDSIQTIYSEALQSAPGSVAQVRECAAQLTRFAKQSGTALIIVGHVTKDGALAGPRVLEHIVDTVLYFEGD
THSSFRLVRAFKNRFGAVNELGVFAMTERGLRGVSNPSAIFLSQHSQRVSGSCVLITQEGTRPLLVEIQALVDAAHSPNP
RRLSVGLEQTRLAMLLAVLHRHAGIVCFDQDVFVNAVGGVKIAEPAADLAILLAIVSSLRDKPLKHGLAVFGEVGLAGEI
RPAPRGQDRLKEAAKLGFTAAIVPKANLPKQSIDGMRVIGVDRIEEALESVRELADS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=243717 CJ010_RS12000 WP_141018244.1 2585834..2587207(+) (radA) [Azoarcus sp. DD4]
ATGGCGAAGGCCAGGACGGTTTTCGTCTGCACCGAATGCGGCGGACAGGCGCTGCGCTGGCAGGGACAGTGCCCGCAGTG
CAGCGCATGGAACACCCTGGTGGAGTCGGTGGCGGAGCAGGGTGGGGCATCGGGCCGCTTTGCCGCGCTGGCCGGGAATA
CCTCGCGCCTGCAGGCGCTGTCGGAACTGCAGCCGCGTGAAGAGCCTCGCACACCGACCGGCATCGACGAGTTCGATCGC
GTGCTCGGCGGCGGCCTCGTGGCGGGCGGGGTGGTGCTGATCGGCGGCGACCCGGGCATCGGCAAGTCGACCCTGTTGCT
GCAGGCGCTGTCCCACCTGGCGGCGGACCAGGCCGCGGTGTACGTCAGCGGCGAAGAGTCCGGCGAACAGGTCGCCCTGC
GCGCTCAGCGCCTGCAACTTGCGCCCACCGGCCTGCAACTGCTCGCGGAGATCAACCTGGAGCGCATCCTCGCCAGCCTG
CGCGACGCCAGGCCGCGCGTGGCGGTGATCGATTCCATCCAGACGATCTATTCCGAGGCGCTGCAGTCCGCGCCCGGTTC
GGTCGCCCAGGTGCGGGAGTGCGCGGCGCAGCTCACCCGTTTCGCCAAGCAGAGCGGCACCGCGCTCATCATCGTCGGCC
ACGTCACCAAGGACGGTGCGCTGGCCGGCCCGCGCGTGCTCGAACACATCGTCGACACGGTGCTCTACTTCGAGGGCGAC
ACGCATTCCAGCTTCCGTCTGGTGCGCGCCTTCAAGAACCGCTTCGGTGCAGTCAATGAGCTTGGTGTGTTCGCGATGAC
CGAGCGCGGCCTGCGCGGTGTGTCCAATCCGTCGGCCATCTTCCTGTCGCAGCACTCGCAGCGGGTTTCGGGCAGTTGCG
TGCTGATCACCCAGGAAGGTACGCGACCGTTGCTGGTCGAGATCCAGGCGCTGGTCGACGCTGCGCACAGCCCGAATCCG
CGCCGGCTGTCGGTCGGTCTGGAGCAGACGAGGCTGGCTATGCTGCTGGCGGTGCTGCACCGCCATGCCGGCATCGTCTG
CTTCGACCAGGATGTGTTCGTCAATGCCGTCGGCGGCGTCAAGATCGCCGAGCCCGCGGCCGATCTCGCGATCCTGCTGG
CCATCGTCTCATCGCTGCGCGACAAGCCGCTCAAGCACGGCTTGGCGGTGTTCGGCGAAGTCGGGCTGGCGGGCGAGATC
AGGCCGGCGCCGCGCGGCCAGGACCGCCTCAAGGAAGCCGCCAAGCTCGGCTTCACCGCAGCCATCGTCCCCAAGGCCAA
CCTGCCCAAGCAGTCCATCGACGGCATGCGGGTGATCGGCGTGGACCGTATCGAGGAAGCGCTGGAGAGCGTTCGCGAGC
TCGCCGATTCCTAG

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(358-450)

AAA domain (AAA_25)

(80-219)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6KT53

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.69

100

0.488
  radA Streptococcus pneumoniae Rx1

46.93

99.781

0.468
  radA Streptococcus pneumoniae D39

46.93

99.781

0.468
  radA Streptococcus pneumoniae TIGR4

46.93

99.781

0.468
  radA Streptococcus pneumoniae R6

46.93

99.781

0.468
  radA Streptococcus mitis SK321

47.033

99.562

0.468
  radA Streptococcus mitis NCTC 12261

46.711

99.781

0.466

Multiple sequence alignment