Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   PSYR_RS02065 Genome accession   NC_007005
Coordinates   425511..427730 (-) Length   739 a.a.
NCBI ID   WP_011266372.1    Uniprot ID   Q4ZZF4
Organism   Pseudomonas syringae pv. syringae B728a     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420511..432730
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYR_RS02045 (Psyr_0394) hslU 420627..421964 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  PSYR_RS02050 (Psyr_0395) hslV 422031..422561 (-) 531 WP_003392345.1 ATP-dependent protease subunit HslV -
  PSYR_RS02055 (Psyr_0396) - 422977..423672 (-) 696 WP_003403056.1 SPOR domain-containing protein -
  PSYR_RS02060 (Psyr_0397) argS 423674..425413 (-) 1740 WP_011266371.1 arginine--tRNA ligase -
  PSYR_RS02065 (Psyr_0398) priA 425511..427730 (-) 2220 WP_011266372.1 primosomal protein N' Machinery gene
  PSYR_RS02070 (Psyr_0399) rpmE 427900..428121 (+) 222 WP_003403048.1 50S ribosomal protein L31 -
  PSYR_RS02075 (Psyr_0400) - 428135..428938 (+) 804 WP_011266373.1 thermonuclease family protein -
  PSYR_RS02080 (Psyr_0401) - 429120..430388 (+) 1269 WP_011266374.1 malic enzyme-like NAD(P)-binding protein -
  PSYR_RS27000 - 430622..430747 (+) 126 Protein_397 IS110 family transposase -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81972.28 Da        Isoelectric Point: 8.5469

>NTDB_id=24316 PSYR_RS02065 WP_011266372.1 425511..427730(-) (priA) [Pseudomonas syringae pv. syringae B728a]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNRDSLNLLLAKELVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALIHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNQRAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSASLPPFSHL
ALLRAEAHKPGQAETFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=24316 PSYR_RS02065 WP_011266372.1 425511..427730(-) (priA) [Pseudomonas syringae pv. syringae B728a]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCCGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGTCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTACCCGCCGACAAGCTGAAGCCGGCTATCGCCCTGCTCGACAGTGAAGCGCCGCTGCCGCCCGCA
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GCTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAAGAGCGCTTCTGGCATGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAAGCCCTGACCACCCTTGCCCAGCACCCGCACGGAGTGGCGCATCAACTGCTC
AGCAAGCTGATGCTTAACAGGGACAGCCTCAACCTGTTGCTGGCCAAGGAGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCACTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGATTCGACAGCTTTCATGCGTTTCTGCTGGCCGGTGTCACCGGCAGCGGCAAGACCGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGATCCACTCGGCGGTCAACGACCGCGAACGGCTGGACGCCTGGC
TGGCGGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGTTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCACTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTGGGCTCGGCCACGCCGTCCCTGGAAAGCCTGCACAACGCCTACACCG
GCCGCTATGGCCTGTTGCGCCTGAATCAGCGCGCGGGCGGCGCACAACAGCCGCGTTTCATGCGCCTGGACGTCAAGAGC
CGACCGCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCTTGGT
GTTCCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAACTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGGCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAACGCCTGGCGATTCTGTTTCC
GGACTACCCCGTGCTGCGTGTCGATCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATCCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACCCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAACTGATCGTGCAGGTGGCAGG
CCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCATCCGTTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAAGCCTTGAGCGAGCGCCGTTCAGCGAGTCTGCCACCGTTTTCACACCTT
GCACTGCTGCGCGCCGAAGCGCACAAGCCGGGACAGGCCGAGACCTTTCTCGATCAGGCCTGCAGCGATGCCGAGCAACT
GGTTGCGCAAATGGCGCTGGGCGGCATAGAGCTGCTGGGCCCGGTTCCGGCCCCGATGGAACGCCGCGCCGGCCGTTATC
GCGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCGCCACTGCATAAATTGCTTGCCACCTGGCTGCTCGCACTGGAGCAA
ATGCCCAGCGGTCGACAGGTCAGATGGTCGCTGGATGTGGATCCCGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q4ZZF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48

Multiple sequence alignment