Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   C9E61_RS00850 Genome accession   NZ_CP028626
Coordinates   162968..163636 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain 137     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 157968..168636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C9E61_RS00820 (C9E61_00845) yicC 157987..158850 (-) 864 WP_000621341.1 YicC/YloC family endoribonuclease -
  C9E61_RS00825 (C9E61_00855) rph 158977..159693 (+) 717 WP_001247093.1 ribonuclease PH -
  C9E61_RS00830 (C9E61_00860) pyrE 159759..160400 (+) 642 WP_000806161.1 orotate phosphoribosyltransferase -
  C9E61_RS00835 (C9E61_00865) slmA 160437..161033 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  C9E61_RS00840 (C9E61_00870) dut 161140..161598 (-) 459 WP_000976070.1 dUTP diphosphatase -
  C9E61_RS00845 (C9E61_00875) coaBC 161576..162796 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  C9E61_RS00850 (C9E61_00880) radC 162968..163636 (+) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  C9E61_RS00855 (C9E61_00885) rpmB 163853..164089 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  C9E61_RS00860 (C9E61_00890) rpmG 164110..164277 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  C9E61_RS00865 (C9E61_00895) mutM 164375..165184 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  C9E61_RS00870 (C9E61_00900) coaD 165223..165702 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  C9E61_RS00875 (C9E61_00905) waaA 165710..166987 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  C9E61_RS00880 (C9E61_00910) rfaQ 167400..168458 (+) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=242859 C9E61_RS00850 WP_001297375.1 162968..163636(+) (radC) [Escherichia coli strain 137]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=242859 C9E61_RS00850 WP_001297375.1 162968..163636(+) (radC) [Escherichia coli strain 137]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACTGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTAGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGACGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GCGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment