Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CJO77_RS14710 Genome accession   NZ_CP022759
Coordinates   3161964..3163463 (+) Length   499 a.a.
NCBI ID   WP_013213612.1    Uniprot ID   A0AAD0S8Y4
Organism   Ralstonia solanacearum strain T98     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3156964..3168463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJO77_RS14685 (CJO77_14645) - 3158142..3159125 (+) 984 WP_013213607.1 KpsF/GutQ family sugar-phosphate isomerase -
  CJO77_RS14690 (CJO77_14650) - 3159158..3159745 (+) 588 WP_013213608.1 KdsC family phosphatase -
  CJO77_RS14695 (CJO77_14655) lptC 3159747..3160379 (+) 633 WP_013213609.1 LPS export ABC transporter periplasmic protein LptC -
  CJO77_RS14700 (CJO77_14660) lptA 3160448..3161044 (+) 597 WP_013213610.1 lipopolysaccharide transport periplasmic protein LptA -
  CJO77_RS14705 (CJO77_14665) lptB 3161078..3161866 (+) 789 WP_013213611.1 LPS export ABC transporter ATP-binding protein -
  CJO77_RS14710 (CJO77_14670) rpoN/rpoN1 3161964..3163463 (+) 1500 WP_013213612.1 RNA polymerase factor sigma-54 Machinery gene
  CJO77_RS14715 (CJO77_14675) hpf 3163515..3163868 (+) 354 WP_013213613.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CJO77_RS14720 (CJO77_14680) ptsN 3164216..3164671 (+) 456 WP_003269869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CJO77_RS14725 (CJO77_14685) hprK 3164742..3165716 (+) 975 WP_011000367.1 HPr(Ser) kinase/phosphatase -
  CJO77_RS14730 (CJO77_14690) - 3165908..3166237 (+) 330 WP_013213614.1 PsiF family protein -
  CJO77_RS14735 (CJO77_14695) rapZ 3166330..3167220 (+) 891 WP_013213615.1 RNase adapter RapZ -
  CJO77_RS14740 (CJO77_14700) - 3167300..3167950 (+) 651 WP_118869889.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55065.44 Da        Isoelectric Point: 4.8496

>NTDB_id=242169 CJO77_RS14710 WP_013213612.1 3161964..3163463(+) (rpoN/rpoN1) [Ralstonia solanacearum strain T98]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGVDGSVSALQSSSATPPPTE
SPPPAANGADHADTAEASYENGSDFDSDYGADRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSLR
DKGLVIFLIESLDDEGYLTGSLDEILADLPAELEAELDELQAALRMLQSFDPAGIGARSAAECLTLQLQRLDSPAKPLAL
AIVNQHLELLAARDYTRLKKALSVDEGALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTAAGWMAQLNPDVMPRL
RINDMYAQILRSSRGESGSANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPLGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=242169 CJO77_RS14710 WP_013213612.1 3161964..3163463(+) (rpoN/rpoN1) [Ralstonia solanacearum strain T98]
ATGAAACAGTCGCTCCAGCTCCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACCATCGAACTGCAACAGGAAGTCGAGCAGGCGCTGACTGAGAACCCGCTGCTTGAGCGCGAGAACGAGTGGC
TCGACACCTCAGCGCGCATCGGCGTCGACGGCTCGGTCAGTGCCCTGCAGAGCAGCAGCGCCACCCCGCCGCCGACCGAA
TCGCCGCCGCCCGCCGCCAACGGCGCGGACCACGCCGACACTGCCGAGGCATCGTACGAAAACGGCAGCGATTTCGACAG
CGACTATGGCGCCGATCGTTCCGACTGGAGCCTCGACGACTTCGCACGCAAACCGCAGTCCGACGAAGACGACCGCGCCC
CGATGCAGCTGCGCGAGGCCGAACCCACGCTGCGCGAATTCCTGATGGAACAGCTGGCGCCGCTCAAGCTGTCCCTGCGC
GACAAAGGCCTCGTCATCTTCCTGATCGAATCGCTGGACGACGAAGGCTACCTGACCGGCTCGCTCGATGAGATCCTCGC
CGACCTGCCGGCCGAACTCGAGGCCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGATCCGGCCG
GCATCGGCGCGCGCTCGGCGGCGGAATGCCTGACGTTGCAGCTGCAGCGCCTGGACTCGCCGGCCAAGCCGCTGGCGCTG
GCCATCGTCAACCAGCATCTCGAACTGCTCGCCGCGCGCGACTACACGCGTCTGAAGAAAGCGCTGTCCGTCGATGAAGG
CGCGCTGAAGGCCGCGCATGAACTGATCCGCTCGCTGGCCCCGTTCCCTGGCCACGCCTTCGGGCGTGCCGAGGCCGACT
TTGTCGTGCCCGACGTGGTGGTGCGTAAAACTGCCGCAGGCTGGATGGCGCAGTTGAACCCCGACGTGATGCCGCGCCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGAGGCGAATCGGGCTCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGCTGGCTGATCAAGAACATCCAACAGCGCTTCGACACCATCCTGCGTGTCTCGCAAGCGATTGTCGAACGTC
AAAAGAGCTTTTTCACGCACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCCTGGGGACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCCGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTCAAAATCCCTGCAGTGAATTTGCGCAAGTCTTTATAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

97.395

100

0.974

Multiple sequence alignment