Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   Kalk_RS15115 Genome accession   NZ_CP022684
Coordinates   3529119..3530483 (+) Length   454 a.a.
NCBI ID   WP_101895042.1    Uniprot ID   A0A2K9LN52
Organism   Ketobacter alkanivorans strain GI5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3524119..3535483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Kalk_RS15080 (Kalk_15055) priB 3524133..3524441 (+) 309 WP_101895036.1 primosomal replication protein N -
  Kalk_RS15085 (Kalk_15060) rpsR 3524464..3524691 (+) 228 WP_101895037.1 30S ribosomal protein S18 -
  Kalk_RS15090 (Kalk_15065) - 3524711..3525580 (+) 870 WP_158643517.1 hypothetical protein -
  Kalk_RS15095 (Kalk_15070) rplI 3525580..3526029 (+) 450 WP_101895039.1 50S ribosomal protein L9 -
  Kalk_RS15100 (Kalk_15075) dnaB 3526250..3527638 (+) 1389 WP_101895040.1 replicative DNA helicase -
  Kalk_RS15105 (Kalk_15080) alr 3527641..3528735 (+) 1095 WP_199767927.1 alanine racemase -
  Kalk_RS15110 (Kalk_15085) - 3528735..3529118 (+) 384 WP_101895041.1 hypothetical protein -
  Kalk_RS15115 (Kalk_15090) radA 3529119..3530483 (+) 1365 WP_101895042.1 DNA repair protein RadA Machinery gene
  Kalk_RS15120 (Kalk_15095) - 3530499..3530867 (-) 369 WP_101895043.1 PilZ domain-containing protein -
  Kalk_RS15125 (Kalk_15100) - 3531202..3531417 (+) 216 WP_101895044.1 PA3496 family putative envelope integrity protein -
  Kalk_RS15130 (Kalk_15105) ettA 3531497..3533167 (-) 1671 WP_101896345.1 energy-dependent translational throttle protein EttA -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48574.81 Da        Isoelectric Point: 6.3676

>NTDB_id=241652 Kalk_RS15115 WP_101895042.1 3529119..3530483(+) (radA) [Ketobacter alkanivorans strain GI5]
MAKAKTQFVCSDCGSVFPKWAGQCVDCGAWNTLTEFNPDRGMSSNRGGKTGWAGEARKVTTMAEVDLVEDPRTTTGTGEL
DRVLGGGLVEGSVVLIGGDPGIGKSTILLQTMTYLASQQAAALYITGEESLQQVALRARRLDLPMDQLRLMSETSIENIL
ATANKEKPRVMVVDSIQTVYSEGLSSAPGGVSQVRECAAALVRFAKQTGTALFLVGHVTKEGALAGPRVLEHMVDSVLYF
EGEQDNRFRMIRAVKNRFGAVNELGVFAMTDKGLREVSNPSAIFLSRYDQPIPGSIVMVTREGSRPLLVEVQALVDDSQL
GNPRRVAVGLDQNRLSMLLAVLHRHGGVATMGMDVFVNVVGGVKVLETGSDLAVLISVVSSLRSMALDSQLIVFGEVGLS
GEIRPVPSGQERLREAAKHGFTRAIVPKGNQPKSPIEGLEVVAVSRLEQALDAL

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=241652 Kalk_RS15115 WP_101895042.1 3529119..3530483(+) (radA) [Ketobacter alkanivorans strain GI5]
ATGGCGAAGGCGAAAACCCAATTTGTCTGCAGCGATTGCGGCAGCGTGTTTCCCAAATGGGCGGGGCAGTGCGTGGATTG
TGGTGCCTGGAACACCTTGACCGAATTCAACCCCGATCGCGGGATGTCGTCCAATCGTGGCGGCAAAACAGGGTGGGCAG
GTGAAGCCCGCAAGGTCACCACCATGGCGGAAGTCGATCTGGTGGAAGACCCCCGTACAACCACCGGTACCGGGGAGCTG
GACCGGGTGCTGGGTGGCGGTTTGGTGGAGGGCTCGGTGGTGCTGATCGGAGGCGACCCCGGCATTGGTAAATCCACCAT
CCTGTTGCAAACCATGACCTATCTGGCGTCACAGCAGGCCGCCGCGCTCTATATAACCGGTGAAGAATCGTTGCAGCAGG
TGGCACTGCGTGCCCGACGGCTGGATTTGCCCATGGATCAGTTGCGGCTGATGTCGGAAACCAGTATCGAGAACATTCTG
GCCACGGCGAATAAAGAGAAGCCAAGGGTTATGGTGGTGGACTCAATCCAGACCGTGTACTCCGAAGGCTTAAGTTCTGC
CCCTGGCGGGGTTTCTCAGGTGCGGGAATGCGCCGCGGCTTTGGTGCGTTTTGCCAAGCAGACAGGGACAGCGCTGTTTC
TGGTGGGGCATGTCACCAAGGAGGGTGCTTTGGCCGGGCCCCGGGTGCTGGAGCACATGGTAGACAGCGTGTTGTATTTT
GAGGGGGAGCAGGATAACCGCTTTCGCATGATTCGGGCGGTTAAGAATCGCTTTGGTGCGGTTAATGAACTGGGCGTATT
TGCCATGACCGATAAAGGGCTGCGGGAAGTCAGCAATCCTTCGGCTATTTTCTTGTCGCGCTATGATCAGCCGATTCCGG
GCAGTATTGTTATGGTGACGCGGGAGGGAAGCCGTCCGTTACTGGTTGAAGTTCAGGCATTGGTGGATGACAGCCAGCTA
GGTAACCCGCGGCGGGTGGCGGTGGGCCTGGATCAGAATAGATTATCAATGCTGTTGGCGGTGCTGCACCGGCACGGTGG
GGTTGCTACCATGGGCATGGATGTATTTGTGAATGTGGTGGGTGGCGTCAAGGTGCTGGAAACCGGTTCTGATTTGGCGG
TGTTGATCTCCGTTGTATCCAGTCTGCGGAGCATGGCGCTGGATAGCCAGCTGATTGTGTTCGGTGAGGTGGGGCTGTCC
GGTGAGATTCGACCTGTGCCCAGCGGGCAGGAGCGTTTGCGGGAGGCGGCCAAGCATGGATTTACACGGGCAATCGTACC
TAAGGGCAACCAGCCCAAGTCGCCCATAGAAGGGCTGGAAGTGGTGGCAGTCTCCCGCCTGGAGCAAGCGCTGGACGCTC
TGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(81-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(366-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K9LN52

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.465

100

0.487
  radA Streptococcus mitis SK321

46.053

100

0.463
  radA Streptococcus pneumoniae Rx1

46.053

100

0.463
  radA Streptococcus pneumoniae TIGR4

46.053

100

0.463
  radA Streptococcus pneumoniae D39

46.053

100

0.463
  radA Streptococcus pneumoniae R6

46.053

100

0.463
  radA Streptococcus mitis NCTC 12261

45.714

100

0.458

Multiple sequence alignment