Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CG018_RS04285 Genome accession   NZ_CP022615
Coordinates   954533..955915 (+) Length   460 a.a.
NCBI ID   WP_114889601.1    Uniprot ID   A0AAD0PZD4
Organism   Gemella sp. ND 6198     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 949533..960915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG018_RS04265 (CG018_04260) clpP 949720..950373 (-) 654 WP_072520146.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  CG018_RS04270 (CG018_04265) - 950949..951362 (+) 414 WP_062172996.1 YjdF family protein -
  CG018_RS04275 (CG018_04270) - 951530..952051 (+) 522 WP_062172997.1 CtsR family transcriptional regulator -
  CG018_RS04280 (CG018_04275) - 952056..954530 (+) 2475 WP_062172998.1 ATP-dependent Clp protease ATP-binding subunit -
  CG018_RS04285 (CG018_04280) radA 954533..955915 (+) 1383 WP_114889601.1 DNA repair protein RadA Machinery gene
  CG018_RS04290 (CG018_04285) - 956312..957379 (+) 1068 WP_062173000.1 PIN/TRAM domain-containing protein -
  CG018_RS04295 (CG018_04290) secG 957454..957684 (+) 231 WP_040461049.1 preprotein translocase subunit SecG -
  CG018_RS04300 (CG018_04295) rnr 957745..959889 (+) 2145 WP_114889602.1 ribonuclease R -
  CG018_RS04305 (CG018_04300) smpB 959886..960341 (+) 456 WP_062173002.1 SsrA-binding protein SmpB -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 51239.29 Da        Isoelectric Point: 9.0045

>NTDB_id=241190 CG018_RS04285 WP_114889601.1 954533..955915(+) (radA) [Gemella sp. ND 6198]
MAKINVKYECNACGYQSLKYLGKCPKCSKWGSLEEVVESKVKSKHTEYRTEETKVQRVEAGKLKTVSTKDVPRTLTDSGE
LNRVLGGGVVGGSLVLLGGDPGIGKSTLLLQISAYLAKEHNVLYVTGEESVRQVKIRADRLKNNTDELYVYAQTDLNLIY
QVVKKIKPQFLVIDSIQTIYNPSLENSPGSITQVRECTQQLMKMAKSLNIAIFIVGHVTKEGTIAGPRMLEHMVDTVLYF
EGESQHSYRILRAVKNRFGSTNEIGIFEMKSSGLHDLENPSRMFLEERSKDLAGATIISTMEGSRPLLVEIQALTTPTAF
NNPRRVSTGLEYNKLVLLMAVLEKRAGYLLQQQDVYVKVSGGVKIDDPAVDLGVIMAVASSFKDIAVDMNDCFIGEVGLT
GEVRRVSRIEQRINEAKKHGFKRVIIPSSNIKNSEFPKEIEIIPVKDIRQCLDIAFKNIF

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=241190 CG018_RS04285 WP_114889601.1 954533..955915(+) (radA) [Gemella sp. ND 6198]
ATGGCAAAGATAAATGTAAAATATGAATGTAATGCCTGTGGTTATCAAAGTTTAAAATATCTGGGTAAATGTCCGAAGTG
TTCAAAGTGGGGATCGTTAGAAGAGGTTGTTGAATCAAAAGTAAAGAGTAAACATACTGAATATCGTACAGAAGAAACAA
AAGTACAACGAGTAGAAGCCGGAAAACTAAAAACGGTTTCTACTAAAGACGTTCCAAGAACGCTGACTGATAGCGGAGAA
TTAAACAGAGTGCTTGGTGGAGGAGTTGTTGGAGGGTCATTAGTATTACTGGGAGGAGATCCCGGCATAGGTAAATCAAC
GCTATTGTTACAAATTTCAGCCTATTTAGCAAAAGAACATAATGTACTTTATGTTACAGGAGAAGAATCTGTAAGACAGG
TGAAAATTAGAGCGGATAGATTAAAAAACAATACGGATGAACTTTATGTTTATGCACAAACAGATCTTAATTTGATTTAT
CAGGTAGTTAAAAAAATAAAACCTCAGTTTTTAGTTATTGATTCAATTCAAACTATATATAATCCAAGTTTGGAAAATTC
TCCCGGTAGTATTACACAGGTGAGGGAATGTACTCAGCAATTAATGAAAATGGCAAAGAGTTTAAATATTGCAATTTTTA
TAGTTGGTCATGTAACAAAAGAAGGTACGATAGCAGGACCTAGAATGTTAGAACACATGGTTGATACCGTCTTGTATTTT
GAAGGTGAGAGTCAGCATAGTTATCGTATTTTACGTGCTGTTAAAAATCGATTCGGTTCAACGAATGAAATCGGTATTTT
TGAAATGAAGTCAAGCGGGCTACATGATTTAGAAAATCCATCGCGAATGTTTTTAGAAGAACGAAGTAAGGACTTAGCAG
GCGCAACGATTATTAGCACAATGGAAGGTAGTCGTCCTCTTTTGGTGGAAATTCAGGCATTAACAACACCGACAGCATTT
AATAATCCGCGACGTGTATCAACCGGATTGGAGTATAATAAGCTGGTATTATTAATGGCTGTATTAGAAAAGCGAGCGGG
GTATTTATTGCAACAGCAAGATGTTTATGTTAAGGTGTCCGGTGGGGTTAAAATAGATGATCCGGCAGTAGATCTTGGTG
TTATAATGGCGGTTGCTTCCAGTTTCAAAGATATTGCGGTTGATATGAATGATTGTTTTATAGGAGAGGTTGGTTTAACT
GGAGAAGTACGGCGTGTTAGCAGAATAGAACAACGTATTAATGAAGCAAAAAAACATGGTTTTAAACGTGTAATTATTCC
TTCGAGCAATATCAAAAACTCAGAATTCCCAAAAGAAATAGAAATAATTCCTGTTAAAGACATTAGACAATGTTTAGACA
TAGCATTTAAAAATATATTTTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(350-456)

AAA domain (AAA_25)

(80-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

57.709

98.696

0.57
  radA Streptococcus pneumoniae Rx1

57.33

99.348

0.57
  radA Streptococcus pneumoniae D39

57.33

99.348

0.57
  radA Streptococcus pneumoniae R6

57.33

99.348

0.57
  radA Streptococcus pneumoniae TIGR4

57.33

99.348

0.57
  radA Streptococcus mitis NCTC 12261

57.33

99.348

0.57
  radA Streptococcus mitis SK321

57.112

99.348

0.567

Multiple sequence alignment