Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   CHR53_RS20490 Genome accession   NZ_CP022572
Coordinates   4175481..4177844 (-) Length   787 a.a.
NCBI ID   WP_066385869.1    Uniprot ID   A0A3T0I298
Organism   Neobacillus mesonae strain H20-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4170481..4182844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR53_RS28315 - 4170554..4170730 (-) 177 WP_164745684.1 hypothetical protein -
  CHR53_RS20470 (CHR53_20560) - 4170895..4172037 (+) 1143 WP_066385862.1 N-acetylmuramoyl-L-alanine amidase -
  CHR53_RS20475 (CHR53_20565) dtd 4172065..4172508 (-) 444 WP_066385864.1 D-aminoacyl-tRNA deacylase -
  CHR53_RS20480 (CHR53_20570) - 4172521..4174716 (-) 2196 WP_066385866.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  CHR53_RS20485 (CHR53_20575) - 4174978..4175490 (-) 513 WP_066385867.1 adenine phosphoribosyltransferase -
  CHR53_RS20490 (CHR53_20580) recJ 4175481..4177844 (-) 2364 WP_066385869.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  CHR53_RS20495 (CHR53_20585) secDF 4177958..4180225 (-) 2268 WP_066385872.1 protein translocase subunit SecDF -
  CHR53_RS20500 (CHR53_20590) - 4180375..4180671 (-) 297 WP_066385874.1 post-transcriptional regulator -
  CHR53_RS20505 (CHR53_20595) spoVB 4180794..4182347 (+) 1554 WP_127488156.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 87966.67 Da        Isoelectric Point: 4.9427

>NTDB_id=240889 CHR53_RS20490 WP_066385869.1 4175481..4177844(-) (recJ) [Neobacillus mesonae strain H20-5]
MLKPKTRWVVRDSDRHKAEQLASELNITPLAASLLVNRGFDSASSARFFLFGKDEFHDPYLFKGMDIAVKRIRTAIDEKE
PILIFGDYDADGVSSTSVLMLTLKDLGADVQFYIPNRFTEGYGPNEAAFRQAAETGIKLIITVDTGIAAVHEANVAKELG
IDLIITDHHEPGPVIPEALAIIHPKLPDSTYPFRELAGVGVAFKLAHALYGRLPEHLFEIAVIGTIADLVSLTGENRLIA
KRGLEKLKVTKNIGLRAIFKLAGVDPMTINEETIGFTIAPRINAVGRLADADLAVDLILCEDSEEAEYLAEEMEGFNKSR
QSIVHTITEEAIAEVESNYPIDDNRVLVIGKEGWNAGVIGIVASKLVEKYYRPAIVLSFDPEKGLAKGSARSIPGFDLFQ
NLSTLRDILPHFGGHPMAAGMTLKLDDVAELREKLNARAFELLTEEDFIPVTYLDEEVHLDEVHLSSLDEIAMLAPFGVD
NPKPKVLINNVEIANIRKIGSEQNHLKITVNDPAGNLDGVGFGLGAVVDEISPASKISLIGELSINEWNNIRKPQIFVQD
IAVNSWQLFDFRGQKRIYSIPSTVPAENRQIIVFNKQYVEKMNSHLGVETIYIEDAEAAKAFDGYHKNIALVDFPPSKEI
LIHLLKGKEPARIYAYFYKASSDFFSTVPTREHFKWYYGFLLKKGPVDLKRYGDDIAKLKGWSRETIIFMSKVFSELDFV
TINNGFITLNNQPQKRDLTDSKTFLQKQAQSALEKDLLFSSFEELKTWFSNIIEESVEIEEEVKQWI

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=240889 CHR53_RS20490 WP_066385869.1 4175481..4177844(-) (recJ) [Neobacillus mesonae strain H20-5]
ATGTTAAAACCGAAAACTAGATGGGTTGTGCGTGATTCTGACCGTCACAAAGCTGAACAGCTTGCATCTGAATTAAACAT
TACTCCTCTTGCTGCATCATTGCTTGTTAACCGTGGATTTGATTCTGCATCCTCCGCACGGTTTTTTTTATTTGGAAAGG
ATGAGTTTCACGATCCCTATTTGTTTAAAGGAATGGATATTGCCGTCAAGCGGATTCGAACTGCGATAGATGAGAAAGAG
CCAATATTGATTTTTGGGGATTATGACGCTGATGGAGTCAGCAGTACGTCTGTATTAATGCTGACACTTAAAGATTTAGG
TGCTGACGTTCAGTTTTATATTCCTAATCGTTTTACAGAAGGTTATGGTCCGAATGAAGCCGCTTTTCGACAGGCGGCCG
AAACCGGTATTAAATTGATCATAACTGTTGACACCGGGATTGCGGCGGTACATGAGGCCAATGTCGCGAAGGAACTGGGA
ATTGATCTCATCATTACTGACCACCACGAACCGGGACCCGTCATTCCGGAAGCACTGGCCATCATTCACCCGAAACTCCC
AGACAGTACGTATCCGTTTCGGGAACTGGCAGGTGTCGGTGTTGCCTTTAAATTAGCCCATGCACTTTATGGCAGACTGC
CGGAGCATTTATTTGAAATAGCCGTTATTGGAACGATTGCAGACCTTGTTTCTTTAACAGGGGAAAATCGTTTAATTGCC
AAAAGAGGTCTGGAAAAATTAAAAGTAACGAAAAATATTGGTCTTCGGGCTATTTTTAAACTAGCGGGTGTCGATCCGAT
GACGATTAACGAAGAAACCATCGGCTTTACGATTGCTCCCCGAATAAACGCTGTTGGGCGGTTAGCGGATGCCGATTTAG
CAGTAGATCTTATTTTATGTGAGGACTCGGAAGAAGCTGAATATCTTGCTGAAGAGATGGAAGGTTTCAATAAATCACGG
CAGTCTATCGTTCATACCATAACAGAGGAAGCAATTGCTGAGGTAGAAAGCAACTATCCAATTGACGATAATAGAGTCCT
AGTTATCGGGAAGGAAGGCTGGAATGCCGGGGTAATTGGCATTGTTGCCTCGAAGCTGGTAGAAAAGTATTATCGTCCTG
CGATTGTTTTAAGCTTTGACCCGGAAAAGGGTTTAGCGAAGGGGTCCGCCCGAAGTATTCCAGGATTTGATCTGTTTCAA
AATTTATCAACGCTAAGAGATATCCTTCCGCATTTTGGAGGCCATCCGATGGCAGCAGGGATGACCTTAAAACTGGATGA
TGTGGCAGAACTGCGTGAAAAATTAAATGCCCGTGCATTTGAATTGCTGACGGAGGAAGATTTTATTCCGGTCACGTATC
TTGATGAAGAAGTGCATTTGGATGAGGTTCATTTATCATCATTGGATGAAATTGCCATGCTTGCGCCGTTTGGGGTAGAT
AATCCGAAGCCCAAGGTACTTATTAATAATGTTGAAATCGCTAATATCCGAAAAATTGGCAGCGAACAAAATCATTTGAA
AATTACTGTTAATGATCCCGCCGGAAATTTGGATGGTGTAGGGTTTGGGCTAGGAGCAGTGGTGGATGAAATTTCTCCCG
CTTCAAAAATATCATTAATCGGAGAATTATCCATCAATGAATGGAACAATATACGTAAACCACAAATTTTTGTCCAAGAC
ATCGCTGTGAATTCGTGGCAGTTATTTGATTTTCGCGGACAGAAGCGGATTTATAGCATTCCATCAACCGTTCCGGCTGA
AAATAGACAGATTATTGTTTTTAATAAACAGTATGTGGAAAAAATGAATTCACATCTTGGTGTGGAGACGATTTATATTG
AGGATGCTGAGGCGGCAAAAGCGTTTGACGGCTACCATAAAAATATTGCCTTAGTAGACTTCCCGCCGTCTAAGGAGATT
CTCATCCACTTATTGAAAGGGAAAGAGCCCGCACGTATTTATGCTTATTTTTACAAAGCATCGAGCGACTTTTTCAGTAC
TGTACCAACACGGGAGCATTTTAAATGGTACTATGGCTTCTTATTGAAAAAAGGACCTGTTGACCTTAAGCGCTATGGAG
ATGATATTGCGAAACTTAAAGGCTGGTCGCGGGAAACGATTATCTTTATGTCAAAGGTGTTTTCTGAATTAGATTTTGTT
ACAATAAACAATGGATTTATTACCTTAAATAATCAACCACAGAAACGCGATTTAACTGACTCGAAAACCTTTCTGCAAAA
GCAGGCACAATCAGCTCTTGAAAAAGATTTATTGTTTTCCTCCTTTGAGGAATTAAAGACTTGGTTTTCGAACATTATCG
AGGAGTCAGTTGAAATTGAGGAGGAAGTTAAACAATGGATTTAA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(455-560)

DHH family (DHH)

(82-224)

DHHA1 domain (DHHA1)

(316-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(567-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T0I298

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

54.257

100

0.543

Multiple sequence alignment