Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CHR53_RS00545 Genome accession   NZ_CP022572
Coordinates   105484..106863 (+) Length   459 a.a.
NCBI ID   WP_066390041.1    Uniprot ID   A0A3T0HS09
Organism   Neobacillus mesonae strain H20-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100484..111863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHR53_RS00525 (CHR53_00525) - 100728..101189 (+) 462 WP_066390029.1 CtsR family transcriptional regulator -
  CHR53_RS00530 (CHR53_00530) - 101267..101815 (+) 549 WP_127484382.1 UvrB/UvrC motif-containing protein -
  CHR53_RS00535 (CHR53_00535) - 101818..102903 (+) 1086 WP_127484384.1 protein arginine kinase -
  CHR53_RS00540 (CHR53_00540) clpC 102909..105353 (+) 2445 WP_127484386.1 ATP-dependent protease ATP-binding subunit ClpC -
  CHR53_RS00545 (CHR53_00545) radA 105484..106863 (+) 1380 WP_066390041.1 DNA repair protein RadA Machinery gene
  CHR53_RS00550 (CHR53_00550) disA 106867..107940 (+) 1074 WP_127484388.1 DNA integrity scanning diadenylate cyclase DisA -
  CHR53_RS00555 (CHR53_00555) - 108145..109233 (+) 1089 WP_127484390.1 PIN/TRAM domain-containing protein -
  CHR53_RS00560 (CHR53_00560) ispD 109270..109953 (+) 684 WP_127484392.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CHR53_RS00565 (CHR53_00565) ispF 110010..110492 (+) 483 WP_127489604.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49608.06 Da        Isoelectric Point: 7.4344

>NTDB_id=240856 CHR53_RS00545 WP_066390041.1 105484..106863(+) (radA) [Neobacillus mesonae strain H20-5]
MVKRKTKFMCQECGYESPKWMGKCPGCGAWNKMVEEVEVTGTARRGAFAHSQGGTIASKPTPITSIEMVSEPRIHTDLAE
LNRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSSQLAGKGHQVLYISGEESLRQTKLRAERLGISSENLLVYAETNLEEI
NRTIEQSNPSFVIIDSIQTVFHPDVTSAPGSVSQVRECTAELMRIGKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLY
FEGERHHTYRILRAVKNRFGSTNEMGIFEMKEFGLEEVENPSEIFLEERSRGASGSTVVASMEGTRPVLVEIQALISPTS
FGNPRRMATGIDHNRVPLLMAVLEKRVGLLLANQDAYVKVAGGVKLDEPAIDLAVAVSIASSFRDQPTRPTDCIIGEVGL
TGEVRRVSRIEQRVQEAAKLGFERVILPANNLGGWKGPKGVELIGVSSVSEALRAALGG

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=240856 CHR53_RS00545 WP_066390041.1 105484..106863(+) (radA) [Neobacillus mesonae strain H20-5]
ATGGTTAAACGGAAAACGAAATTTATGTGTCAGGAGTGCGGGTATGAATCCCCAAAATGGATGGGAAAATGCCCGGGCTG
TGGTGCTTGGAATAAAATGGTGGAGGAAGTAGAGGTAACCGGTACAGCAAGAAGGGGGGCTTTTGCCCATTCCCAAGGCG
GAACAATTGCTTCTAAGCCTACACCTATTACTTCGATTGAAATGGTCAGTGAACCTAGGATCCATACGGATTTAGCAGAA
CTAAACCGGGTTCTTGGCGGCGGTGTAGTAAAAGGTTCATTAGTACTAATCGGCGGTGACCCGGGGATTGGCAAGTCTAC
ACTCCTTTTGCAGGTCTCCTCACAACTTGCGGGGAAAGGCCATCAGGTTTTATACATATCGGGAGAGGAATCGTTACGGC
AGACAAAATTAAGGGCTGAACGATTAGGAATCTCCTCGGAAAACCTGCTTGTTTATGCGGAAACAAATCTTGAGGAAATT
AATCGTACGATTGAGCAGAGTAATCCAAGCTTTGTTATCATTGACTCGATTCAAACAGTTTTCCATCCGGATGTGACGTC
GGCACCGGGCAGTGTATCCCAGGTTCGTGAATGTACCGCGGAATTAATGAGAATCGGGAAAACAAAGGGAATTGCTATTT
TTATTGTCGGCCATGTCACAAAGGAAGGGGCGATTGCTGGACCACGCCTGTTAGAGCATATGGTTGATACGGTACTTTAT
TTTGAGGGAGAAAGACACCATACCTATCGTATCCTCAGAGCTGTGAAAAACCGCTTTGGCTCAACAAATGAAATGGGCAT
TTTTGAAATGAAGGAATTTGGCTTGGAAGAAGTGGAAAATCCATCAGAGATTTTCCTCGAAGAACGGTCACGAGGAGCAT
CCGGGTCCACTGTCGTTGCCTCCATGGAAGGAACGCGGCCGGTTCTGGTGGAGATTCAAGCATTGATCTCCCCGACAAGT
TTCGGTAATCCTAGAAGAATGGCGACGGGAATTGACCATAACCGGGTGCCGTTATTGATGGCAGTGCTCGAAAAGCGGGT
AGGACTTCTGCTGGCTAACCAGGATGCCTACGTAAAAGTTGCCGGCGGTGTTAAATTAGATGAACCGGCCATCGATCTAG
CAGTTGCCGTCAGTATTGCCTCAAGCTTTAGAGACCAGCCGACAAGACCGACAGACTGCATTATTGGTGAGGTCGGATTA
ACAGGGGAAGTAAGACGGGTATCCCGGATTGAACAGCGTGTCCAAGAGGCCGCGAAACTGGGCTTTGAGCGGGTTATCCT
GCCAGCCAATAATCTAGGCGGCTGGAAGGGGCCGAAGGGAGTCGAACTCATTGGTGTATCGTCAGTCAGCGAGGCACTAA
GAGCAGCATTAGGGGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-223)

Subunit ChlI of Mg-chelatase (ChlI)

(364-431)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T0HS09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

77.391

100

0.776
  radA Streptococcus mitis NCTC 12261

62.009

99.782

0.619
  radA Streptococcus pneumoniae Rx1

62.009

99.782

0.619
  radA Streptococcus pneumoniae D39

62.009

99.782

0.619
  radA Streptococcus pneumoniae R6

62.009

99.782

0.619
  radA Streptococcus pneumoniae TIGR4

62.009

99.782

0.619
  radA Streptococcus mitis SK321

61.79

99.782

0.617

Multiple sequence alignment