Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   SPA_RS15020 Genome accession   NC_006511
Coordinates   3069305..3070285 (-) Length   326 a.a.
NCBI ID   WP_001055655.1    Uniprot ID   A0A5Z0RZB6
Organism   Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3064305..3075285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPA_RS14990 (SPA2956) endA 3064763..3065470 (+) 708 WP_000286124.1 deoxyribonuclease I -
  SPA_RS14995 (SPA2957) rsmE 3065547..3066278 (+) 732 WP_001222488.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SPA_RS15000 (SPA2958) gshB 3066298..3067245 (+) 948 WP_000593248.1 glutathione synthase -
  SPA_RS15005 (SPA2959) - 3067461..3068024 (+) 564 WP_001053177.1 YqgE/AlgH family protein -
  SPA_RS15010 (SPA2960) ruvX 3068024..3068440 (+) 417 WP_001285491.1 Holliday junction resolvase RuvX -
  SPA_RS15015 (SPA2961) - 3068487..3069173 (-) 687 WP_000098319.1 IclR family transcriptional regulator domain-containing protein -
  SPA_RS15020 (SPA2962) pilT 3069305..3070285 (-) 981 WP_001055655.1 type IV pilus twitching motility protein PilT Machinery gene
  SPA_RS15025 (SPA2963) - 3070303..3071007 (+) 705 WP_000997790.1 YggS family pyridoxal phosphate-dependent enzyme -
  SPA_RS15030 (SPA2964) - 3071026..3071592 (+) 567 WP_001094848.1 YggT family protein -
  SPA_RS15035 (SPA2965) yggU 3071589..3071879 (+) 291 WP_001277203.1 DUF167 family protein YggU -
  SPA_RS15040 (SPA2966) - 3071887..3072480 (+) 594 WP_001174769.1 XTP/dITP diphosphatase -
  SPA_RS15045 (SPA2967) hemW 3072473..3073609 (+) 1137 WP_001096530.1 radical SAM family heme chaperone HemW -
  SPA_RS15050 (SPA2968) - 3073700..3074707 (-) 1008 WP_000252189.1 DUF1202 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36139.35 Da        Isoelectric Point: 7.8164

>NTDB_id=24079 SPA_RS15020 WP_001055655.1 3069305..3070285(-) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMETAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFKHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREVGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQEGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=24079 SPA_RS15020 WP_001055655.1 3069305..3070285(-) (pilT) [Salmonella enterica subsp. enterica serovar Paratyphi A str. ATCC 9150]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGACCGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ACGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCAAACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCCACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAGTCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTGCGTGAAGATCCCGATGTCATTTTGCTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGTCAACTGGCGGGTAGTCTGCGCGCTGTACTATCGCAAAAACTGGAGGT
CGACAGGCAGGAGGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGCGCAGGGACGACTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(78-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5Z0RZB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.93

100

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.93

100

0.491
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Pseudomonas stutzeri DSM 10701

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.969

97.546

0.439
  pilT Legionella pneumophila strain ERS1305867

44.969

97.546

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.453

97.546

0.414
  pilU Vibrio cholerae strain A1552

38.871

97.853

0.38
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment