Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BSG32_RS00175 Genome accession   NZ_CP022552
Coordinates   29159..30175 (-) Length   338 a.a.
NCBI ID   WP_021449761.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain MAVP-R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 24159..35175
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSG32_RS00150 (BSG32_00150) aroK 25019..25537 (-) 519 WP_005381319.1 shikimate kinase AroK -
  BSG32_RS00155 (BSG32_00155) pilQ 25726..27474 (-) 1749 WP_025442303.1 type IV pilus secretin PilQ Machinery gene
  BSG32_RS00160 (BSG32_00160) pilP 27506..28021 (-) 516 WP_021449759.1 pilus assembly protein PilP Machinery gene
  BSG32_RS00165 (BSG32_00165) pilO 28011..28604 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  BSG32_RS00170 (BSG32_00170) pilN 28597..29175 (-) 579 WP_021449760.1 PilN domain-containing protein Machinery gene
  BSG32_RS00175 (BSG32_00175) pilM 29159..30175 (-) 1017 WP_021449761.1 type IV pilus biogenesis protein PilM Machinery gene
  BSG32_RS00180 (BSG32_00180) - 30318..32888 (+) 2571 WP_025622219.1 penicillin-binding protein 1A -
  BSG32_RS00185 (BSG32_00185) oxyR 32944..33852 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  BSG32_RS00190 (BSG32_00190) - 34023..34751 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37748.26 Da        Isoelectric Point: 6.8322

>NTDB_id=240647 BSG32_RS00175 WP_021449761.1 29159..30175(-) (pilM) [Vibrio parahaemolyticus strain MAVP-R]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=240647 BSG32_RS00175 WP_021449761.1 29159..30175(-) (pilM) [Vibrio parahaemolyticus strain MAVP-R]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGTCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACGTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment