Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CGZ77_RS03515 Genome accession   NZ_CP022527
Coordinates   705850..707934 (-) Length   694 a.a.
NCBI ID   WP_009424990.1    Uniprot ID   L1P4H5
Organism   Neisseria sp. KEM232     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700850..712934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ77_RS03500 (CGZ77_03505) - 700893..702050 (+) 1158 WP_009424994.1 surface lipoprotein assembly modifier -
  CGZ77_RS12620 - 702122..702298 (+) 177 WP_198142874.1 surface lipoprotein assembly modifier -
  CGZ77_RS03505 (CGZ77_03510) - 702580..704457 (-) 1878 WP_009424992.1 monovalent cation:proton antiporter-2 (CPA2) family protein -
  CGZ77_RS03510 (CGZ77_03515) trpB 704524..705726 (-) 1203 WP_009424991.1 tryptophan synthase subunit beta -
  CGZ77_RS03515 (CGZ77_03520) pilQ 705850..707934 (-) 2085 WP_009424990.1 type IV pilus secretin PilQ Machinery gene
  CGZ77_RS03520 (CGZ77_03525) - 707954..708469 (-) 516 WP_009424989.1 pilus assembly protein PilP -
  CGZ77_RS03525 (CGZ77_03530) - 708485..709114 (-) 630 WP_009424988.1 type 4a pilus biogenesis protein PilO -
  CGZ77_RS03530 (CGZ77_03535) - 709115..709756 (-) 642 WP_009424987.1 PilN domain-containing protein -
  CGZ77_RS03535 (CGZ77_03540) pilM 709759..710856 (-) 1098 WP_094030945.1 type IV pilus assembly protein PilM -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 75437.37 Da        Isoelectric Point: 9.6702

>NTDB_id=240306 CGZ77_RS03515 WP_009424990.1 705850..707934(-) (pilQ) [Neisseria sp. KEM232]
MNKVKTINSIFAILGLSFIGHSVYAGNITNINVSSMPNNQKIIKIKFDKDAVLPVASPSVSPTQFVLNFPTTGVGLSQPV
LEYEDSLLNRVMAAQQADGQAKVVVGLNKNGRYTTEVKGNEVWIRINESVGVQANVKAGQQQVANSASTVDFRKSGKSEG
IVDISVPGFQGQPDIKQNSSSITITLKNHPLPTQAQRNLDVSDFGTPVRNVVMKRIGNDTQVVIKNQGNWSYNHKAAGGR
ISVEISPKTSVEVNGLQSNKSKSFRGNRVSFDFQDVDVRTILQILAKESGMNIVASDSVTGKMTLTLKDVPWDQALDLVM
QARGLDMRRSGNIINVAPRSELLARDKEILTQQREIDELGPLYSRTFQLKYKNVEEFRKILKLDDSSSSSDNSKTILSNR
GSALIDPATNTLIITDNSSVLQKFEKLIAELDVPARQVMVEARIVEASDGFSRELGVKFGYAGSNGKNSWGSNWANAQAN
NNTYGNALQNNTNRTWTLDPNVNLPTSAATSSLALVHALSSGALALELSAQQAQQKAKIISSPRVLTQDRKEAVIESGTE
VPYQEASSSGATSTSFKKAVLGMTVTPQITPDGQIIMDIKVNKDSVNRNCGASEPCIDTKRLDTRAMVEDGGTLILGGIY
EEENSEGVSKVPLLGDVPVLGNLFKHRSRNNSKKELLIFITPRIVDNLGSTLRY

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=240306 CGZ77_RS03515 WP_009424990.1 705850..707934(-) (pilQ) [Neisseria sp. KEM232]
ATGAATAAAGTTAAAACCATCAACAGTATATTTGCAATATTGGGATTGTCATTTATCGGTCATTCGGTGTATGCGGGAAA
CATAACCAACATCAATGTTTCCTCTATGCCGAATAATCAAAAAATTATTAAAATTAAATTTGATAAGGATGCGGTTTTAC
CCGTTGCGTCCCCAAGTGTTTCGCCTACACAGTTTGTTCTTAATTTTCCTACTACAGGTGTTGGCTTGTCTCAACCTGTT
TTGGAATATGAAGATTCCCTATTAAATCGGGTGATGGCTGCTCAGCAGGCGGACGGACAGGCTAAAGTTGTTGTCGGTTT
GAACAAAAACGGACGTTATACAACTGAAGTGAAAGGTAATGAAGTTTGGATTCGGATTAACGAATCTGTAGGTGTTCAAG
CAAATGTCAAAGCCGGCCAGCAACAGGTTGCAAACTCCGCTTCGACTGTTGATTTTAGAAAATCCGGTAAATCCGAAGGT
ATTGTCGATATTTCTGTTCCCGGTTTTCAGGGGCAGCCTGACATTAAGCAAAACAGCAGCAGCATTACAATTACTTTAAA
AAACCATCCTTTGCCGACTCAAGCCCAACGCAATTTGGATGTAAGCGATTTTGGTACGCCCGTCCGTAATGTGGTCATGA
AAAGAATTGGTAATGACACGCAGGTTGTCATTAAAAACCAAGGAAATTGGTCGTATAACCACAAGGCGGCCGGAGGACGA
ATTTCAGTAGAAATTTCTCCTAAAACTTCTGTTGAAGTAAACGGTTTGCAATCCAATAAGTCGAAATCTTTTAGAGGCAA
TCGGGTTTCCTTCGATTTCCAAGATGTAGATGTGCGTACCATCTTGCAGATTTTGGCGAAAGAGTCAGGCATGAATATCG
TTGCCAGCGATAGTGTCACCGGTAAGATGACTCTTACTTTGAAAGATGTGCCTTGGGATCAAGCTCTTGATTTGGTGATG
CAGGCTCGCGGTTTGGATATGCGTCGTTCGGGCAATATCATCAATGTTGCACCAAGATCTGAGCTGTTGGCTAGAGATAA
GGAAATACTGACTCAGCAGCGCGAAATTGATGAATTGGGTCCGCTGTATTCTCGTACATTCCAATTGAAATACAAAAATG
TGGAAGAGTTCCGCAAGATTTTGAAATTGGATGACAGTAGCAGTTCTTCCGACAATAGTAAGACTATTTTGAGCAATAGA
GGTAGCGCGTTAATTGATCCTGCTACCAATACTTTGATTATCACGGATAATTCTTCTGTTTTGCAAAAATTTGAGAAATT
GATTGCAGAATTGGACGTGCCTGCACGTCAAGTAATGGTAGAAGCTAGGATTGTTGAGGCTTCCGACGGTTTTTCCCGTG
AACTGGGTGTGAAATTCGGTTATGCCGGCTCTAACGGAAAAAACAGCTGGGGCAGCAACTGGGCAAATGCACAAGCTAAT
AACAATACTTACGGTAACGCGCTTCAGAACAATACCAATAGAACTTGGACGCTGGATCCGAATGTGAATTTACCGACAAG
TGCTGCGACATCCAGCCTGGCTTTGGTGCATGCCCTTTCTTCAGGCGCGTTGGCATTAGAGCTGTCCGCACAGCAAGCCC
AACAAAAGGCTAAAATTATTTCTTCGCCGCGGGTGTTAACCCAAGACCGCAAAGAGGCCGTTATTGAGTCCGGTACGGAA
GTTCCTTATCAGGAAGCTTCTTCCAGTGGCGCAACTTCAACCTCCTTCAAAAAAGCAGTTTTGGGAATGACGGTTACTCC
GCAGATTACACCTGACGGTCAGATTATTATGGACATTAAGGTAAACAAAGACTCTGTTAACCGTAACTGTGGTGCTTCCG
AGCCCTGTATCGATACCAAGCGCTTGGATACCAGAGCCATGGTGGAAGACGGCGGCACTCTGATTTTGGGTGGCATTTAT
GAAGAAGAAAACTCTGAAGGTGTCAGCAAAGTTCCGTTGTTGGGTGATGTGCCTGTGTTGGGTAACCTGTTCAAACACCG
CTCCCGCAACAACAGCAAAAAAGAGCTGCTGATCTTTATCACTCCGCGCATTGTCGATAATCTCGGCAGTACGCTGCGTT
ACTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(365-437)

Bacterial type II and III secretion system protein (Secretin)

(531-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L1P4H5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

53.203

100

0.55
  pilQ Neisseria meningitidis MC58

57.451

80.259

0.461
  pilQ Neisseria meningitidis M1080

57.658

79.971

0.461

Multiple sequence alignment