Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CGZ77_RS00365 Genome accession   NZ_CP022527
Coordinates   75159..76541 (-) Length   460 a.a.
NCBI ID   WP_009427352.1    Uniprot ID   L1NJC2
Organism   Neisseria sp. KEM232     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 70159..81541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ77_RS00340 (CGZ77_00340) - 70262..71038 (-) 777 WP_009427358.1 ferredoxin--NADP reductase -
  CGZ77_RS00345 (CGZ77_00345) pcnB 71129..72466 (-) 1338 WP_036496758.1 polynucleotide adenylyltransferase PcnB -
  CGZ77_RS00350 (CGZ77_00350) - 72720..73232 (+) 513 WP_009427355.1 thioredoxin family protein -
  CGZ77_RS00355 (CGZ77_00355) - 73321..73749 (+) 429 WP_009427354.1 EamA family transporter -
  CGZ77_RS00360 (CGZ77_00360) - 73829..75052 (-) 1224 WP_009427353.1 aspartate kinase -
  CGZ77_RS00365 (CGZ77_00365) radA 75159..76541 (-) 1383 WP_009427352.1 DNA repair protein RadA Machinery gene
  CGZ77_RS00370 (CGZ77_00370) - 76844..78979 (+) 2136 WP_198344877.1 M3 family metallopeptidase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49508.07 Da        Isoelectric Point: 7.6300

>NTDB_id=240303 CGZ77_RS00365 WP_009427352.1 75159..76541(-) (radA) [Neisseria sp. KEM232]
MAKAPKTIYQCSECGGTAVKWQGKCPHCGEWNTLQESVAAPEPKNARFQSWAAESTQVQELSRVSAVEVPREATGMGELD
RVLGGGLVDGAVILLGGDPGIGKSTLLLQTIALMAKKRKVLYVSGEESAQQVALRAQRLGLNAEGVNLLAEIRLEAVQAA
LKQHHPGVVVIDSIQTMYSDQITSAPGSVSQVRECAAQFTRIAKQMGIAMIFVGHVTKDGAIAGPRVLEHMVDTVLYFEG
DQHSNYRMIRAIKNRFGAANELGVFAMTETGLKGVSNPSAIFLASYRDDVAGSCVLVTQEGSRPLLVEIQALVDDAHGFT
PKRLTVGLEQNRLSMLLAVLNRHAGIACFDQDVFLNAVGGVKIGEPASDLAVILAMVSSFRNKALPEKMVAFGEIGLSGE
VRPVARGQERLKEAEKLGFKRAIVPKANLPRNAKEFPNLQIYGVERLDEAVRLCRDLADG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=240303 CGZ77_RS00365 WP_009427352.1 75159..76541(-) (radA) [Neisseria sp. KEM232]
ATGGCAAAAGCCCCCAAAACCATCTACCAATGCTCCGAATGCGGCGGCACCGCCGTCAAATGGCAGGGCAAATGCCCGCA
TTGCGGCGAGTGGAACACCTTGCAGGAAAGCGTCGCCGCGCCCGAGCCGAAAAACGCGCGCTTCCAATCTTGGGCGGCGG
AGAGTACGCAGGTGCAGGAACTCTCGCGCGTCAGCGCGGTGGAAGTGCCGCGCGAAGCCACCGGCATGGGCGAGCTCGAC
CGCGTGCTCGGCGGCGGGCTGGTGGACGGCGCGGTGATTCTGCTGGGCGGCGACCCGGGCATCGGCAAGTCCACCCTGCT
GCTGCAAACCATTGCCCTGATGGCGAAAAAACGCAAAGTGCTGTATGTGTCGGGTGAAGAATCCGCGCAACAGGTCGCCC
TGCGCGCCCAGCGTTTGGGGCTGAATGCCGAAGGCGTGAACCTGCTGGCGGAAATCCGTCTGGAAGCCGTTCAGGCTGCC
TTAAAGCAGCACCACCCGGGCGTGGTGGTTATCGACTCGATCCAAACCATGTATTCCGACCAAATCACATCCGCCCCCGG
CAGCGTGTCGCAGGTGCGCGAGTGCGCCGCCCAGTTCACCCGCATCGCCAAGCAGATGGGCATCGCCATGATTTTTGTCG
GACACGTTACCAAAGACGGCGCCATCGCCGGCCCGCGCGTGCTGGAACACATGGTGGACACCGTGCTTTATTTTGAAGGC
GACCAGCATTCCAACTACCGCATGATCCGCGCCATCAAAAACCGCTTCGGCGCGGCCAACGAATTGGGCGTGTTCGCCAT
GACCGAAACGGGCCTGAAAGGCGTGTCCAACCCGTCCGCCATTTTTCTCGCCAGCTACCGCGACGACGTGGCAGGCTCGT
GCGTGCTGGTAACGCAGGAAGGCAGCCGCCCGCTCTTGGTGGAAATCCAAGCGCTGGTGGACGACGCGCACGGCTTCACG
CCCAAACGTTTAACAGTAGGGCTGGAACAAAACCGCCTGTCCATGCTCTTGGCGGTGCTCAACCGCCACGCCGGCATCGC
CTGCTTCGACCAAGACGTGTTTCTCAACGCCGTCGGCGGCGTGAAAATCGGCGAACCGGCTTCCGACTTGGCGGTGATAC
TGGCGATGGTATCGAGCTTCCGCAACAAAGCCCTGCCCGAGAAAATGGTCGCCTTCGGCGAGATTGGTCTCAGCGGCGAA
GTGCGCCCCGTCGCCCGCGGGCAGGAGCGGCTCAAAGAAGCGGAAAAACTCGGCTTCAAACGCGCCATCGTCCCCAAAGC
CAACCTGCCGCGCAACGCCAAAGAGTTTCCCAATCTGCAGATTTACGGCGTGGAGCGGCTGGATGAAGCGGTGCGGCTGT
GTCGGGATTTGGCAGACGGGTGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(70-220)

Subunit ChlI of Mg-chelatase (ChlI)

(366-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L1NJC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.781

99.13

0.493
  radA Streptococcus mitis NCTC 12261

47.577

98.696

0.47
  radA Streptococcus pneumoniae D39

47.682

98.478

0.47
  radA Streptococcus pneumoniae TIGR4

47.682

98.478

0.47
  radA Streptococcus pneumoniae R6

47.682

98.478

0.47
  radA Streptococcus pneumoniae Rx1

47.682

98.478

0.47
  radA Streptococcus mitis SK321

47.566

98.261

0.467

Multiple sequence alignment