Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CGU46_RS15345 Genome accession   NZ_CP022525
Coordinates   3207467..3207916 (+) Length   149 a.a.
NCBI ID   WP_021264133.1    Uniprot ID   A0A509JKL4
Organism   Pseudomonas aeruginosa strain Ocean-1175     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3202467..3212916
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGU46_RS15320 (CGU46_15320) yacG 3202630..3202830 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  CGU46_RS15325 (CGU46_15325) coaE 3202827..3203438 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  CGU46_RS15330 (CGU46_15330) pilD 3203435..3204307 (-) 873 WP_021264129.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  CGU46_RS15335 (CGU46_15335) pilC 3204311..3205531 (-) 1221 WP_023101565.1 type 4a pilus biogenesis protein PilC Machinery gene
  CGU46_RS15340 (CGU46_15340) pilB 3205535..3207235 (-) 1701 WP_021264132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CGU46_RS15345 (CGU46_15345) pilA 3207467..3207916 (+) 450 WP_021264133.1 pilin Machinery gene
  CGU46_RS15355 (CGU46_15355) ltrA 3208533..3209954 (+) 1422 WP_011805433.1 group II intron reverse transcriptase/maturase -
  CGU46_RS15360 (CGU46_15360) nadC 3210038..3210886 (-) 849 WP_021264134.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15537.82 Da        Isoelectric Point: 7.7818

>NTDB_id=240258 CGU46_RS15345 WP_021264133.1 3207467..3207916(+) (pilA) [Pseudomonas aeruginosa strain Ocean-1175]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGTNIKIGTDPSTATETYV
GVAANANKLGLIDVKIADTGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=240258 CGU46_RS15345 WP_021264133.1 3207467..3207916(+) (pilA) [Pseudomonas aeruginosa strain Ocean-1175]
ATGAAAGCTCAAAAAGGCTTTACTTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCGATTGCCAT
TCCCCAGTACCAGAACTATGTGGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAATCCGCTGAAGACCACTGTTG
AAGAGTCTCTGTCGCGTGGAATTGCTGGTACCAATATTAAAATTGGTACTGATCCATCTACTGCCACCGAAACATATGTT
GGGGTTGCTGCGAATGCCAACAAGCTGGGCTTGATTGATGTAAAGATTGCTGATACTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACTTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAATCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A509JKL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

68.667

100

0.691
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.358

100

0.47
  pilA Acinetobacter baumannii strain A118

47.297

99.329

0.47
  comP Acinetobacter baylyi ADP1

43.59

100

0.456
  pilA Vibrio cholerae C6706

42.949

100

0.45
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.949

100

0.45
  pilA Vibrio cholerae strain A1552

42.949

100

0.45
  pilA Vibrio parahaemolyticus RIMD 2210633

45.578

98.658

0.45
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.455

95.973

0.436
  pilA Acinetobacter nosocomialis M2

41.429

93.96

0.389
  pilA2 Legionella pneumophila str. Paris

39.189

99.329

0.389
  pilA2 Legionella pneumophila strain ERS1305867

38.514

99.329

0.383

Multiple sequence alignment