Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CG015_RS12910 Genome accession   NZ_CP022468
Coordinates   2786084..2787094 (-) Length   336 a.a.
NCBI ID   WP_094165236.1    Uniprot ID   -
Organism   Vibrio anguillarum strain MHK3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2781084..2792094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG015_RS12885 (CG015_12895) aroK 2781951..2782472 (-) 522 WP_010317691.1 shikimate kinase AroK -
  CG015_RS12890 (CG015_12900) pilQ 2782659..2784374 (-) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  CG015_RS12895 (CG015_12905) pilP 2784398..2784919 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  CG015_RS12900 (CG015_12910) pilO 2784906..2785499 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  CG015_RS12905 (CG015_12915) pilN 2785492..2786094 (-) 603 WP_017047271.1 PilN domain-containing protein Machinery gene
  CG015_RS12910 (CG015_12920) pilM 2786084..2787094 (-) 1011 WP_094165236.1 type IV pilus assembly protein PilM Machinery gene
  CG015_RS12915 (CG015_12925) - 2787244..2789742 (+) 2499 WP_129532792.1 penicillin-binding protein 1A -
  CG015_RS12920 (CG015_12930) oxyR 2789779..2790669 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  CG015_RS12925 (CG015_12935) - 2790816..2791544 (+) 729 WP_129532793.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37396.95 Da        Isoelectric Point: 7.9013

>NTDB_id=239736 CG015_RS12910 WP_094165236.1 2786084..2787094(-) (pilM) [Vibrio anguillarum strain MHK3]
MGKSLVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQRGKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=239736 CG015_RS12910 WP_094165236.1 2786084..2787094(-) (pilM) [Vibrio anguillarum strain MHK3]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAAATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCGGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGTAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGTGCAGATACTCCGATGTTGGCGCAAGAGATCTCGCGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCGAGGTAAGCAGTCTGGGCAGAGTTTTAGCACCGCAGCT
GGTCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.976

100

0.53

Multiple sequence alignment