Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CGZ81_RS03305 Genome accession   NZ_CP022449
Coordinates   640717..642093 (+) Length   458 a.a.
NCBI ID   WP_003549080.1    Uniprot ID   Q5FM36
Organism   Lactobacillus acidophilus strain ATCC 53544     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 635717..647093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CGZ81_RS10205 (CGZ81_03275) - 636366..636548 (+) 183 WP_003549074.1 hypothetical protein -
  CGZ81_RS03280 (CGZ81_03280) - 636550..637227 (+) 678 WP_021721658.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  CGZ81_RS03285 (CGZ81_03285) - 637332..638267 (+) 936 WP_003549076.1 AAA family ATPase -
  CGZ81_RS03290 (CGZ81_03290) pepC 638318..639667 (-) 1350 WP_003549077.1 aminopeptidase C -
  CGZ81_RS03295 (CGZ81_03295) - 639738..640034 (-) 297 WP_003549078.1 hypothetical protein -
  CGZ81_RS03300 (CGZ81_03300) - 640166..640717 (+) 552 WP_011254121.1 dUTP diphosphatase -
  CGZ81_RS03305 (CGZ81_03305) radA 640717..642093 (+) 1377 WP_003549080.1 DNA repair protein RadA Machinery gene
  CGZ81_RS03310 (CGZ81_03310) gltX 642171..643670 (+) 1500 WP_003549081.1 glutamate--tRNA ligase -
  CGZ81_RS03315 (CGZ81_03315) cysS 643768..645201 (+) 1434 WP_003549082.1 cysteine--tRNA ligase -
  CGZ81_RS03320 (CGZ81_03320) - 645194..645637 (+) 444 WP_003549083.1 Mini-ribonuclease 3 -
  CGZ81_RS03325 (CGZ81_03325) rlmB 645624..646376 (+) 753 WP_011254123.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CGZ81_RS03330 (CGZ81_03330) - 646516..647052 (+) 537 WP_011254124.1 DNA-directed RNA polymerase subunit sigma -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50253.83 Da        Isoelectric Point: 6.9044

>NTDB_id=239590 CGZ81_RS03305 WP_003549080.1 640717..642093(+) (radA) [Lactobacillus acidophilus strain ATCC 53544]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETEEVQKRSTKATASRLIQKTGVNEPVKLDKIKAEKEERISTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDLAEKHKVLYVSGEESANQIKLRADRLGIGQSNMLLYPESDMHDI
REQINDVKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMDAITVFVIGHVTKEGAIAGPKIMEHMVDTVLY
FEGDEHHTYRILHSVKNRFGAANEIGMFEMVNEGLKEVTNPSAIFLDQRLPNSTGSAVVVSLEGTRPLLAEIQALVTPTA
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYTDKEISPTDCFVGEVGL
TGEIRRVDKIEARVKEAAKVGFKRIFIPKHNMYQGLTNNGIEVIPVSSIPQALKLVFN

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=239590 CGZ81_RS03305 WP_003549080.1 640717..642093(+) (radA) [Lactobacillus acidophilus strain ATCC 53544]
ATGGCAAGGGTAAAAACTCAATATAAATGTCGCTCCTGTGGTTACATTTCTGCTAGTTATTTAGGTAGATGTCCTAACTG
TGGAGCATGGAATCAATTTGAAAAAGAAACTGAAGAAGTACAAAAACGTTCAACAAAAGCTACAGCTAGCCGTTTAATCC
AAAAAACTGGCGTAAATGAACCAGTAAAGCTAGATAAAATTAAAGCGGAAAAAGAAGAGAGAATTTCTACTAAATCAGAA
GAATTAAATCGTGTTTTAGGTGGCGGAATTGTACCTGGATCGCTTGTATTAATCGGTGGAGATCCTGGAATTGGTAAATC
AACTTTAATGTTGCAAATTATGAGTGATTTGGCAGAAAAGCATAAAGTATTATACGTTTCCGGAGAAGAATCAGCTAATC
AAATTAAGCTAAGAGCTGATCGGTTAGGAATCGGACAGAGTAACATGCTTTTATATCCTGAAAGTGATATGCATGATATT
CGTGAACAAATTAATGATGTTAAACCTGATTTTGTAGTTATTGATTCAATTCAAACAATGAATGAACCTAGCTTAGATTC
TATGACAGGTTCTGCCTCACAGGTTCGTGAAGTAACTAGTGAATTGATGAAAATCGCAAAAATGGATGCAATTACTGTTT
TTGTAATTGGACATGTAACTAAAGAGGGTGCAATTGCGGGACCTAAAATTATGGAACATATGGTAGATACTGTTCTATAT
TTTGAAGGGGATGAGCACCATACTTATAGAATTTTACATTCTGTGAAAAATCGTTTTGGTGCTGCTAATGAAATTGGCAT
GTTTGAAATGGTGAATGAAGGGTTAAAAGAAGTAACTAATCCGTCAGCTATTTTTCTTGATCAAAGATTACCAAATTCGA
CTGGTTCAGCAGTAGTTGTTTCTTTGGAAGGAACACGCCCGCTTTTGGCAGAAATTCAGGCTTTGGTTACGCCAACGGCA
TTTGGTTATGCAAAAAGAACAACGTCAGGGATTGATTATAATAGGGCTGCTCTTTTACTGGCAGTACTTGAAAAGCGTGG
TAATTTGATGTTGCAAAACCAAGATGTATATTTGACTGCTACTGGTGGGATTAAGTTGAATGAGCCTGCGATTGATTTGG
CGATTGCAATGTCTGTTGCATCTAGTTATACAGACAAAGAAATTTCACCAACTGATTGTTTTGTAGGTGAAGTAGGTTTA
ACGGGAGAAATCCGTAGAGTAGATAAAATTGAAGCTAGAGTAAAAGAAGCTGCTAAAGTTGGTTTTAAGCGAATTTTTAT
TCCTAAACACAATATGTATCAAGGATTAACTAATAACGGAATAGAAGTTATTCCAGTTTCAAGCATTCCGCAAGCGTTAA
AATTAGTTTTTAATTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

AAA domain (AAA_25)

(66-223)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5FM36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

57.549

99.782

0.574
  radA Streptococcus pneumoniae D39

57.549

99.782

0.574
  radA Streptococcus pneumoniae R6

57.549

99.782

0.574
  radA Streptococcus pneumoniae TIGR4

57.549

99.782

0.574
  radA Streptococcus mitis NCTC 12261

57.549

99.782

0.574
  radA Streptococcus mitis SK321

57.549

99.782

0.574
  radA Bacillus subtilis subsp. subtilis str. 168

56.264

99.345

0.559

Multiple sequence alignment