Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Asalp_RS20915 Genome accession   NZ_CP022426
Coordinates   4462253..4463692 (-) Length   479 a.a.
NCBI ID   WP_021140209.1    Uniprot ID   -
Organism   Aeromonas salmonicida subsp. pectinolytica 34mel     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4457253..4468692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Asalp_RS20885 (Asalp_40870) - 4457526..4458266 (+) 741 WP_021140213.1 KDGP aldolase family protein -
  Asalp_RS20890 (Asalp_40880) - 4458370..4460274 (+) 1905 WP_021140212.1 PRD domain-containing protein -
  Asalp_RS20895 (Asalp_40890) npr 4460293..4460565 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  Asalp_RS20900 (Asalp_40900) rapZ 4460586..4461452 (-) 867 WP_021140211.1 RNase adapter RapZ -
  Asalp_RS20905 (Asalp_40910) ptsN 4461495..4461941 (-) 447 WP_021140210.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Asalp_RS20910 (Asalp_40920) hpf 4461944..4462231 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  Asalp_RS20915 (Asalp_40930) rpoN/rpoN1 4462253..4463692 (-) 1440 WP_021140209.1 RNA polymerase factor sigma-54 Machinery gene
  Asalp_RS20920 (Asalp_40940) lptB 4463758..4464483 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  Asalp_RS20925 (Asalp_40950) lptA 4464487..4465014 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  Asalp_RS20930 (Asalp_40960) lptC 4465001..4465561 (-) 561 WP_021140208.1 LPS export ABC transporter periplasmic protein LptC -
  Asalp_RS20935 (Asalp_40970) kdsC 4465558..4466112 (-) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  Asalp_RS20940 (Asalp_40980) - 4466112..4467107 (-) 996 WP_034524103.1 KpsF/GutQ family sugar-phosphate isomerase -
  Asalp_RS20945 (Asalp_40990) mlaF 4467315..4468115 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53905.87 Da        Isoelectric Point: 4.3744

>NTDB_id=239317 Asalp_RS20915 WP_021140209.1 4462253..4463692(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. pectinolytica 34mel]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEVQETSPEEPVDDSQLDSSDAMAQDRMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=239317 Asalp_RS20915 WP_021140209.1 4462253..4463692(-) (rpoN/rpoN1) [Aeromonas salmonicida subsp. pectinolytica 34mel]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGAGG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCGGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCTGCCATTGCTCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATTGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTCGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCACGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTGGTCAAGAAGGGCAACAAATGGGTCGTTGAACTCAACCCGG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACTAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.972

100

0.489

Multiple sequence alignment