Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VITFI_RS15475 Genome accession   NZ_CP022423
Coordinates   3287429..3288331 (-) Length   300 a.a.
NCBI ID   WP_232476635.1    Uniprot ID   -
Organism   Vitreoscilla filiformis strain ATCC 15551     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3282429..3293331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VITFI_RS15460 (VITFI_CDS3098) - 3283421..3285136 (-) 1716 WP_089417742.1 PAS domain-containing sensor histidine kinase -
  VITFI_RS15465 (VITFI_CDS3099) - 3285153..3285953 (-) 801 WP_089418202.1 inner membrane protein YpjD -
  VITFI_RS15470 (VITFI_CDS3100) ffh 3286026..3287405 (+) 1380 WP_089417743.1 signal recognition particle protein -
  VITFI_RS15475 (VITFI_CDS3101) pilA 3287429..3288331 (-) 903 WP_232476635.1 signal recognition particle-docking protein FtsY Machinery gene
  VITFI_RS15485 (VITFI_CDS3102) miaB 3288988..3290331 (+) 1344 WP_089417745.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  VITFI_RS15490 (VITFI_CDS3103) - 3290347..3290646 (-) 300 WP_089417746.1 hypothetical protein -
  VITFI_RS15495 (VITFI_CDS3104) hemA 3290779..3292089 (+) 1311 WP_089417747.1 glutamyl-tRNA reductase -
  VITFI_RS15500 (VITFI_CDS3105) prfA 3292102..3293184 (+) 1083 WP_089417748.1 peptide chain release factor 1 -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 32227.05 Da        Isoelectric Point: 6.5062

>NTDB_id=239293 VITFI_RS15475 WP_232476635.1 3287429..3288331(-) (pilA) [Vitreoscilla filiformis strain ATCC 15551]
MARLRKGLSRTGSAIAQVFTGTQLTEEMYEELETALLQADAGVKATEFLLKDLRRRVKEAKATDPQQVRELLIEAITELL
GPLERGLVVGEYTPTVMMVVGVNGAGKTTSIGKLTHHLAAADCRVLLAAADTFRAAAREQLSVWADRNRVEIVSQEGGDP
ASVSFDAVNAGRARGCDVVIADTAGRLPTQLHLMEELRKVRRVIAKADATAPHEVLLVVDGNTGQNALNQVKAFDEALTL
TGLVVTKLDGTAKGGVLAAIALWSRERQLGSIPVYFIGVGEKLEDLQTFSAREFAKALLS

Nucleotide


Download         Length: 903 bp        

>NTDB_id=239293 VITFI_RS15475 WP_232476635.1 3287429..3288331(-) (pilA) [Vitreoscilla filiformis strain ATCC 15551]
ATGGCCCGATTGCGTAAGGGTTTGTCGCGCACCGGCTCGGCCATCGCCCAGGTGTTCACGGGCACCCAGCTCACCGAGGA
AATGTACGAGGAGCTGGAAACCGCTTTGTTGCAAGCCGACGCGGGCGTGAAAGCCACCGAGTTCTTGCTGAAAGATCTGC
GCCGGCGTGTCAAGGAAGCCAAGGCCACCGACCCTCAACAGGTGCGCGAACTGCTCATCGAGGCCATCACCGAACTGTTG
GGCCCGCTGGAGCGGGGGCTGGTTGTGGGTGAGTACACACCCACGGTGATGATGGTGGTGGGCGTCAACGGTGCGGGCAA
GACCACCAGCATCGGCAAACTCACCCACCATTTGGCGGCAGCCGACTGCCGTGTGCTGCTGGCCGCAGCGGACACGTTCC
GCGCCGCTGCACGGGAGCAACTGTCGGTGTGGGCGGATCGCAACCGCGTGGAAATCGTCTCCCAAGAGGGCGGCGATCCG
GCGTCGGTGAGTTTTGACGCCGTCAACGCTGGCCGCGCACGCGGCTGCGACGTGGTGATCGCCGATACCGCTGGCCGGTT
GCCGACGCAGTTGCACCTGATGGAAGAGCTGCGCAAAGTGCGCCGCGTCATCGCCAAGGCGGACGCCACCGCGCCGCACG
AAGTGCTCTTGGTCGTCGATGGCAACACCGGCCAAAACGCGCTCAACCAAGTGAAGGCGTTTGATGAAGCGTTGACGCTG
ACCGGCCTCGTCGTCACCAAGTTGGACGGCACCGCCAAGGGTGGCGTGCTGGCGGCGATCGCGTTGTGGTCACGCGAGCG
CCAGCTCGGTTCGATCCCGGTTTACTTCATCGGTGTGGGCGAGAAGCTGGAAGACCTGCAAACCTTCAGCGCCCGCGAGT
TTGCGAAAGCGCTGCTGAGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(94-299)

SRP54-type protein, helical bundle domain (SRP54_N)

(13-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

56.766

100

0.573

Multiple sequence alignment