Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C6668_RS21330 Genome accession   NZ_CP028166
Coordinates   4091931..4093316 (-) Length   461 a.a.
NCBI ID   WP_001025210.1    Uniprot ID   -
Organism   Escherichia coli strain CFSAN064036     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4086931..4098316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6668_RS21300 (C6668_21290) mutT 4087145..4087534 (+) 390 WP_000736046.1 8-oxo-dGTP diphosphatase MutT -
  C6668_RS21305 (C6668_21295) yacG 4087866..4088063 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  C6668_RS21310 (C6668_21300) zapD 4088073..4088816 (-) 744 WP_001194734.1 cell division protein ZapD -
  C6668_RS21315 (C6668_21305) coaE 4088816..4089436 (-) 621 WP_001269510.1 dephospho-CoA kinase -
  C6668_RS26435 - 4089461..4089505 (+) 45 WP_120795372.1 protein YacM -
  C6668_RS21320 (C6668_21310) guaC 4089661..4090704 (+) 1044 WP_001217349.1 GMP reductase -
  C6668_RS21325 (C6668_21315) hofC 4090739..4091941 (-) 1203 WP_000157273.1 protein transport protein HofC -
  C6668_RS21330 (C6668_21320) pilB 4091931..4093316 (-) 1386 WP_001025210.1 type II secretion system protein GspE Machinery gene
  C6668_RS21335 (C6668_21325) pilA 4093326..4093766 (-) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  C6668_RS21345 (C6668_21335) nadC 4093969..4094862 (-) 894 WP_001135149.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C6668_RS21350 (C6668_21340) ampD 4094950..4095501 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C6668_RS21355 (C6668_21345) ampE 4095498..4096352 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  C6668_RS21360 (C6668_21350) - 4096382..4097332 (-) 951 WP_001154422.1 family 43 glycosylhydrolase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50622.21 Da        Isoelectric Point: 6.5405

>NTDB_id=239017 C6668_RS21330 WP_001025210.1 4091931..4093316(-) (pilB) [Escherichia coli strain CFSAN064036]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVQGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=239017 C6668_RS21330 WP_001025210.1 4091931..4093316(-) (pilB) [Escherichia coli strain CFSAN064036]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGCCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCAGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGATTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATAGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTCGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36

Multiple sequence alignment