Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   CCX84_RS27630 Genome accession   NZ_CP022345
Coordinates   5191204..5192553 (+) Length   449 a.a.
NCBI ID   WP_000879428.1    Uniprot ID   W8YBC3
Organism   Bacillus thuringiensis strain c25     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5186204..5197553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX84_RS27610 - 5186472..5186786 (+) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  CCX84_RS27615 - 5186935..5188362 (+) 1428 WP_000499480.1 C40 family peptidase -
  CCX84_RS30890 - 5189411..5189620 (+) 210 WP_269769369.1 hypothetical protein -
  CCX84_RS27625 - 5189767..5190837 (+) 1071 WP_061139627.1 endo-1,4-beta-xylanase -
  CCX84_RS27630 comFA 5191204..5192553 (+) 1350 WP_000879428.1 ATP-dependent helicase ComFA Machinery gene
  CCX84_RS27635 - 5192553..5193257 (+) 705 WP_033685746.1 ComF family protein -
  CCX84_RS27640 cspC 5193384..5193581 (+) 198 WP_001990088.1 cold shock protein CspC -
  CCX84_RS27645 raiA 5193904..5194446 (+) 543 WP_000671186.1 ribosome-associated translation inhibitor RaiA -
  CCX84_RS27650 - 5194851..5195324 (+) 474 WP_000511548.1 hypothetical protein -
  CCX84_RS27655 - 5195442..5196011 (+) 570 WP_061139628.1 hypothetical protein -
  CCX84_RS27660 - 5196039..5196827 (+) 789 WP_000120044.1 hypothetical protein -
  CCX84_RS27665 - 5196814..5197509 (+) 696 WP_000466510.1 hypothetical protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51010.89 Da        Isoelectric Point: 9.9712

>NTDB_id=238563 CCX84_RS27630 WP_000879428.1 5191204..5192553(+) (comFA) [Bacillus thuringiensis strain c25]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=238563 CCX84_RS27630 WP_000879428.1 5191204..5192553(+) (comFA) [Bacillus thuringiensis strain c25]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGGGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8YBC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372

Multiple sequence alignment