Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CCX84_RS05480 Genome accession   NZ_CP022345
Coordinates   965897..966331 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain c25     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 960897..971331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCX84_RS05455 - 961811..962974 (-) 1164 WP_000434600.1 aminotransferase A -
  CCX84_RS05460 - 963097..963297 (-) 201 WP_000929280.1 hypothetical protein -
  CCX84_RS05465 kinB 963608..964882 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  CCX84_RS05470 - 964921..965670 (-) 750 WP_000388471.1 DUF3967 domain-containing protein -
  CCX84_RS05480 nucA/comI 965897..966331 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  CCX84_RS05485 comJ 966368..966757 (+) 390 WP_000424030.1 competence protein ComJ -
  CCX84_RS05490 metE 966796..969084 (-) 2289 WP_061138974.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CCX84_RS05495 - 969596..970153 (-) 558 WP_000062063.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=238526 CCX84_RS05480 WP_000811494.1 965897..966331(+) (nucA/comI) [Bacillus thuringiensis strain c25]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=238526 CCX84_RS05480 WP_000811494.1 965897..966331(+) (nucA/comI) [Bacillus thuringiensis strain c25]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGTGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment