Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   CFN60_RS10715 Genome accession   NZ_CP022341
Coordinates   2188530..2189138 (+) Length   202 a.a.
NCBI ID   WP_014418235.1    Uniprot ID   -
Organism   Bacillus velezensis strain 157     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2183530..2194138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN60_RS10675 (CFN60_10670) - 2184550..2185056 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  CFN60_RS10680 (CFN60_10675) - 2185195..2185608 (+) 414 WP_007612169.1 Hsp20/alpha crystallin family protein -
  CFN60_RS10685 (CFN60_10680) - 2185638..2186105 (-) 468 WP_014418231.1 YppG family protein -
  CFN60_RS10690 (CFN60_10685) - 2186292..2186474 (+) 183 WP_003153532.1 YppF family protein -
  CFN60_RS10695 (CFN60_10690) - 2186513..2186881 (-) 369 WP_014418232.1 YppE family protein -
  CFN60_RS10700 (CFN60_10695) - 2186927..2187172 (-) 246 WP_014418233.1 DUF5446 family protein -
  CFN60_RS10705 (CFN60_10700) sspM 2187378..2187482 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  CFN60_RS10710 (CFN60_10705) - 2187526..2188488 (-) 963 WP_022553046.1 DUF2515 domain-containing protein -
  CFN60_RS10715 (CFN60_10710) recU 2188530..2189138 (+) 609 WP_014418235.1 Holliday junction resolvase RecU Machinery gene
  CFN60_RS10720 (CFN60_10715) - 2189177..2191960 (+) 2784 WP_022553045.1 PBP1A family penicillin-binding protein -
  CFN60_RS10725 (CFN60_10720) - 2192038..2192529 (-) 492 WP_022553044.1 YpoC family protein -
  CFN60_RS10730 (CFN60_10725) nth 2192526..2193185 (-) 660 WP_022553043.1 endonuclease III -
  CFN60_RS10735 (CFN60_10730) dnaD 2193204..2193902 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=238450 CFN60_RS10715 WP_014418235.1 2188530..2189138(+) (recU) [Bacillus velezensis strain 157]
MIRYPNGKTFQPNKTVSSQNSQKRTHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWDRKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=238450 CFN60_RS10715 WP_014418235.1 2188530..2189138(+) (recU) [Bacillus velezensis strain 157]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGACCCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGACAGAAAGGAG
AAGAACGGCAGAAAATCGATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

91.457

98.515

0.901

Multiple sequence alignment