Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   CFN60_RS08260 Genome accession   NZ_CP022341
Coordinates   1620588..1621487 (+) Length   299 a.a.
NCBI ID   WP_014417764.1    Uniprot ID   -
Organism   Bacillus velezensis strain 157     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1615588..1626487
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN60_RS08240 (CFN60_08240) - 1616279..1617982 (+) 1704 WP_014417761.1 hypothetical protein -
  CFN60_RS08245 (CFN60_08245) - 1617979..1618260 (+) 282 WP_014417762.1 FlhB-like flagellar biosynthesis protein -
  CFN60_RS08250 (CFN60_08250) sucC 1618435..1619592 (+) 1158 WP_003154283.1 ADP-forming succinate--CoA ligase subunit beta -
  CFN60_RS08255 (CFN60_08255) sucD 1619621..1620523 (+) 903 WP_014417763.1 succinate--CoA ligase subunit alpha -
  CFN60_RS08260 (CFN60_08260) dprA 1620588..1621487 (+) 900 WP_014417764.1 DNA-processing protein DprA Machinery gene
  CFN60_RS08265 (CFN60_08265) topA 1621669..1623744 (+) 2076 WP_014417765.1 type I DNA topoisomerase -
  CFN60_RS08270 (CFN60_08270) trmFO 1623809..1625116 (+) 1308 WP_012117550.1 FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO -
  CFN60_RS08275 (CFN60_08275) xerC 1625186..1626103 (+) 918 WP_007409774.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 32850.04 Da        Isoelectric Point: 8.4593

>NTDB_id=238445 CFN60_RS08260 WP_014417764.1 1620588..1621487(+) (dprA) [Bacillus velezensis strain 157]
MDQASRCLMVCSINQIISPSLLLKWWKADHSLSFLPDPHPLTVLSEGKTAPEAIFREIERKEPELDEVLSDYRRKGITVI
PISSSRYPTWLKAIYDPPAVLYAKGNTLLLEKGRKIGIVGTRKPTEDGIKAAGHLSAELSKKGWVIVSGLASGIDGLSHK
ASIRAKGLTIGVIAGGFHHIYPRENLLLAEYMAEHHLLLSEHPPETKPKKWHFPMRNRIISGLSEGIVVVQGKEKSGSLI
TAYQALDQGREVFAVPGSIFNPYSGGPIKLIQEGAKAVLCAEDIDGELTARCVQYTEPF

Nucleotide


Download         Length: 900 bp        

>NTDB_id=238445 CFN60_RS08260 WP_014417764.1 1620588..1621487(+) (dprA) [Bacillus velezensis strain 157]
TTGGATCAAGCATCGCGCTGTTTAATGGTCTGCAGTATTAATCAAATCATTTCCCCGTCTCTTCTATTAAAATGGTGGAA
AGCTGACCACTCTCTGTCTTTTTTACCGGATCCGCATCCATTAACTGTTTTATCAGAAGGGAAAACAGCCCCGGAAGCAA
TTTTTCGGGAAATAGAGCGCAAGGAACCGGAACTTGATGAAGTTCTGTCCGATTACCGCCGCAAAGGCATTACTGTCATT
CCGATTTCATCAAGCCGCTATCCAACATGGCTTAAAGCGATTTATGATCCGCCGGCTGTCTTGTATGCAAAAGGGAACAC
GCTGCTTCTTGAAAAAGGCAGAAAAATCGGGATTGTAGGAACGCGGAAACCGACGGAAGACGGAATAAAAGCGGCTGGGC
ATCTTTCCGCCGAACTCTCAAAAAAAGGCTGGGTCATTGTAAGCGGGCTTGCATCCGGTATAGACGGATTGTCTCATAAG
GCGAGCATCAGGGCAAAAGGGCTTACGATCGGCGTGATAGCCGGCGGATTCCATCATATCTATCCCCGGGAAAATCTCCT
GTTAGCAGAATACATGGCTGAACACCATCTCCTACTCTCAGAACATCCTCCTGAAACAAAGCCGAAAAAATGGCACTTTC
CGATGAGAAACCGCATAATCAGCGGACTAAGTGAAGGAATTGTGGTCGTGCAGGGAAAAGAAAAAAGCGGTTCATTAATC
ACAGCTTACCAGGCCCTCGATCAAGGCAGAGAGGTATTTGCCGTTCCGGGTTCCATATTTAATCCATATTCCGGAGGACC
TATAAAACTCATTCAAGAAGGGGCGAAAGCTGTATTATGCGCAGAGGATATTGACGGAGAGCTGACCGCCCGATGCGTTC
AGTATACGGAACCCTTTTGA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(73-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Bacillus subtilis subsp. subtilis str. 168

70.569

100

0.706
  dprA Lactococcus lactis subsp. cremoris KW2

41.288

88.294

0.365

Multiple sequence alignment