Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   CFN60_RS05860 Genome accession   NZ_CP022341
Coordinates   1137724..1139553 (+) Length   609 a.a.
NCBI ID   WP_003155028.1    Uniprot ID   -
Organism   Bacillus velezensis strain 157     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1132724..1144553
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN60_RS05835 (CFN60_05835) - 1133387..1133965 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  CFN60_RS05840 (CFN60_05840) spx 1134144..1134539 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  CFN60_RS05845 (CFN60_05845) - 1134596..1135252 (-) 657 WP_014417421.1 TerC family protein -
  CFN60_RS05850 (CFN60_05850) mecA 1135528..1136184 (+) 657 WP_038461347.1 adaptor protein MecA Regulator
  CFN60_RS05855 (CFN60_05855) - 1136336..1137496 (+) 1161 WP_014417422.1 competence protein CoiA family protein -
  CFN60_RS05860 (CFN60_05860) pepF 1137724..1139553 (+) 1830 WP_003155028.1 oligoendopeptidase F Regulator
  CFN60_RS20055 (CFN60_05865) - 1139591..1139758 (-) 168 WP_003155026.1 hypothetical protein -
  CFN60_RS05870 (CFN60_05870) spxH 1140045..1140947 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  CFN60_RS05875 (CFN60_05875) - 1140944..1141342 (-) 399 WP_025649585.1 thiol management oxidoreductase -
  CFN60_RS05880 (CFN60_05880) - 1141571..1142257 (-) 687 WP_022553553.1 lytic transglycosylase domain-containing protein -
  CFN60_RS05885 (CFN60_05885) - 1142262..1142834 (-) 573 WP_021494986.1 CYTH domain-containing protein -
  CFN60_RS05890 (CFN60_05890) - 1142959..1143324 (+) 366 WP_012117290.1 hypothetical protein -
  CFN60_RS05895 (CFN60_05895) yjbM 1143352..1143987 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69799.43 Da        Isoelectric Point: 5.2024

>NTDB_id=238439 CFN60_RS05860 WP_003155028.1 1137724..1139553(+) (pepF) [Bacillus velezensis strain 157]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKKYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYASILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRGTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=238439 CFN60_RS05860 WP_003155028.1 1137724..1139553(+) (pepF) [Bacillus velezensis strain 157]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATCCCGAATTTATCTTCTTTTAAAGGAA
CGCTTGCTCATTCGGCTGACAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGCAAACTTTAT
GCATACGCGCATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTCATTC
TGGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGCCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGCGTTTTAAATAACGCCGATAT
TACATTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTTCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCTGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTGAAAAAAGATAATTTTTACGCCCGTGTGAAAAAATATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATCCCTGAAGAAGTTTACGATAACCTTATTAAAACGATAAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTCGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACGCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTGATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAGGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATCTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAGAAGCTGAACGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment