Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CFH90_RS03055 Genome accession   NZ_CP022298
Coordinates   603725..604525 (+) Length   266 a.a.
NCBI ID   WP_126035299.1    Uniprot ID   A0A3Q8XC45
Organism   Acinetobacter johnsonii strain IC001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 598725..609525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFH90_RS03025 (CFH90_03010) - 598936..599352 (+) 417 WP_004983505.1 hypothetical protein -
  CFH90_RS03030 (CFH90_03015) - 599493..600773 (+) 1281 WP_126035293.1 glycosyltransferase family 4 protein -
  CFH90_RS03035 (CFH90_03020) - 600966..601517 (+) 552 WP_126035295.1 phosphatase PAP2 family protein -
  CFH90_RS03040 (CFH90_03025) iscX 601608..601808 (+) 201 WP_004697668.1 Fe-S cluster assembly protein IscX -
  CFH90_RS03045 (CFH90_03030) ndk 601919..602350 (+) 432 WP_004983508.1 nucleoside-diphosphate kinase -
  CFH90_RS03050 (CFH90_03035) rlmN 602473..603708 (+) 1236 WP_126035297.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CFH90_RS03055 (CFH90_03040) pilF 603725..604525 (+) 801 WP_126035299.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CFH90_RS03060 (CFH90_03045) - 604516..605292 (+) 777 WP_004697676.1 helix-turn-helix domain-containing protein -
  CFH90_RS03065 (CFH90_03050) ispG 605311..606426 (+) 1116 WP_126035301.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CFH90_RS03070 (CFH90_03055) hisS 606423..607718 (+) 1296 WP_126035303.1 histidine--tRNA ligase -
  CFH90_RS03075 (CFH90_03060) - 607746..608444 (+) 699 WP_005401704.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30020.97 Da        Isoelectric Point: 9.1263

>NTDB_id=238190 CFH90_RS03055 WP_126035299.1 603725..604525(+) (pilF) [Acinetobacter johnsonii strain IC001]
MKKPVSKLYLALTLGVSAWYLVGCQTVNTKGDPSKAVQVRTQLAAEYIKSNDYDAAKRTLDQALDIDSRDASANMMMGVL
LQREGSPQNVEKAERYFKHAIAAEPKNAQARNNYGTYLYQIGRYNDAIDQLQVAGSTLGYDQRYRALENLGRAYLQVGRV
AEAEAAFKQALQVNSGAYLAMIEMAEISYLRQQNAEATQYYEQFVRAVGEKNLDARALWIGIRIARASHDTMGSQVLVNQ
LRALYPDSQEYKRYLQLQYTTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=238190 CFH90_RS03055 WP_126035299.1 603725..604525(+) (pilF) [Acinetobacter johnsonii strain IC001]
TTGAAAAAACCCGTTTCTAAACTTTACCTTGCGCTGACACTGGGTGTGTCAGCGTGGTACTTGGTGGGTTGCCAAACCGT
GAATACGAAAGGTGATCCAAGTAAAGCAGTACAAGTTAGAACGCAGTTGGCGGCTGAATATATTAAGTCGAATGATTACG
ATGCTGCGAAACGCACATTGGATCAGGCATTAGACATCGACTCTCGTGATGCATCTGCAAATATGATGATGGGCGTTTTG
TTACAGCGTGAAGGCAGTCCACAAAATGTGGAAAAGGCAGAGCGTTATTTTAAGCATGCAATTGCGGCTGAGCCAAAAAA
TGCACAGGCTCGTAACAACTACGGTACGTATTTATATCAAATTGGGCGTTATAATGACGCGATTGATCAACTTCAAGTTG
CAGGTTCAACTTTAGGGTATGATCAACGTTATCGAGCATTGGAAAATTTAGGTCGTGCGTATCTACAAGTAGGTCGAGTG
GCGGAAGCAGAAGCAGCTTTTAAACAAGCCCTACAGGTAAACTCAGGTGCCTATCTTGCCATGATCGAAATGGCGGAAAT
TTCATATTTACGTCAGCAAAATGCTGAAGCTACGCAGTACTATGAACAGTTTGTGCGAGCGGTTGGCGAGAAAAACTTAG
ATGCACGTGCGCTTTGGATTGGGATCCGAATTGCGCGAGCAAGTCATGACACGATGGGTTCGCAAGTACTGGTCAACCAG
CTACGTGCATTGTATCCAGACAGTCAAGAATACAAACGTTATTTGCAATTACAGTACACTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_19)

(89-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q8XC45

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

67.91

100

0.684
  pilF Acinetobacter baylyi ADP1

64.662

100

0.647

Multiple sequence alignment