Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   CFN13_RS07550 Genome accession   NZ_CP022291
Coordinates   1542161..1543231 (-) Length   356 a.a.
NCBI ID   WP_000776403.1    Uniprot ID   -
Organism   Staphylococcus aureus strain EDCC5464     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537161..1548231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN13_RS07510 (CFN13_07515) gcvPA 1537338..1538684 (-) 1347 WP_000019688.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CFN13_RS07515 (CFN13_07520) gcvT 1538704..1539795 (-) 1092 WP_000093355.1 glycine cleavage system aminomethyltransferase GcvT -
  CFN13_RS07520 (CFN13_07525) - 1539954..1540478 (-) 525 WP_001015122.1 shikimate kinase -
  CFN13_RS07525 (CFN13_07530) - 1540468..1540614 (-) 147 WP_001789879.1 hypothetical protein -
  CFN13_RS07530 (CFN13_07535) comGF 1540711..1541208 (-) 498 WP_029697681.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFN13_RS07535 (CFN13_07540) comGE 1541126..1541425 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CFN13_RS07540 (CFN13_07545) comGD 1541412..1541858 (-) 447 WP_075583651.1 competence type IV pilus minor pilin ComGD Machinery gene
  CFN13_RS07545 (CFN13_07550) comGC 1541836..1542147 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  CFN13_RS07550 (CFN13_07555) comGB 1542161..1543231 (-) 1071 WP_000776403.1 competence type IV pilus assembly protein ComGB Machinery gene
  CFN13_RS07555 (CFN13_07560) comGA 1543203..1544177 (-) 975 WP_000697215.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN13_RS07560 (CFN13_07565) - 1544229..1544852 (-) 624 WP_001223006.1 MBL fold metallo-hydrolase -
  CFN13_RS07565 (CFN13_07570) - 1544849..1545178 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CFN13_RS07570 (CFN13_07575) - 1545178..1546164 (-) 987 WP_000161315.1 ROK family glucokinase -
  CFN13_RS07575 (CFN13_07580) - 1546161..1546364 (-) 204 WP_000087561.1 YqgQ family protein -
  CFN13_RS07580 (CFN13_07585) - 1546345..1547808 (-) 1464 WP_100185099.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41487.99 Da        Isoelectric Point: 10.1030

>NTDB_id=238096 CFN13_RS07550 WP_000776403.1 1542161..1543231(-) (comGB) [Staphylococcus aureus strain EDCC5464]
MKLQWINTFKLHSKKRQLSKAQQIDLLLNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=238096 CFN13_RS07550 WP_000776403.1 1542161..1543231(-) (comGB) [Staphylococcus aureus strain EDCC5464]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTTAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCGATTTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTATTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTGGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(29-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment