Detailed information    

insolico Bioinformatically predicted

Overview

Name   recQ   Type   Machinery gene
Locus tag   I3O_RS24010 Genome accession   NZ_CP028117
Coordinates   4513623..4515452 (+) Length   609 a.a.
NCBI ID   WP_001345114.1    Uniprot ID   -
Organism   Escherichia coli O111 str. RM9322     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4508623..4520452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3O_RS23975 (I3O_23965) - 4508901..4509752 (-) 852 WP_001059701.1 YPO3983 family protein -
  I3O_RS23980 (I3O_23970) - 4509933..4510085 (+) 153 Protein_4427 CorA family divalent cation transporter -
  I3O_RS23990 (I3O_23980) - 4510709..4511008 (+) 300 WP_000526415.1 hypothetical protein -
  I3O_RS23995 (I3O_23985) rarD 4511055..4511937 (-) 883 Protein_4429 EamA family transporter RarD -
  I3O_RS24000 (I3O_23990) yigI 4511989..4512456 (-) 468 WP_001277142.1 acyl-CoA thioesterase YigI -
  I3O_RS24005 (I3O_23995) pldA 4512621..4513490 (+) 870 WP_001259700.1 phospholipase A -
  I3O_RS24010 (I3O_24000) recQ 4513623..4515452 (+) 1830 WP_001345114.1 ATP-dependent DNA helicase RecQ Machinery gene
  I3O_RS24015 (I3O_24005) rhtC 4515516..4516136 (+) 621 WP_000928824.1 threonine export protein RhtC -
  I3O_RS24020 (I3O_24010) rhtB 4516198..4516818 (-) 621 WP_000171710.1 homoserine/homoserine lactone efflux protein -
  I3O_RS24025 (I3O_24015) pldB 4516929..4517951 (+) 1023 WP_000487654.1 lysophospholipase L2 -
  I3O_RS24030 (I3O_24020) yigL 4517959..4518759 (+) 801 WP_000285352.1 sugar/pyridoxal phosphate phosphatase YigL -
  I3O_RS24035 (I3O_24025) bioP 4518835..4519734 (+) 900 WP_001196238.1 biotin transporter -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 68391.56 Da        Isoelectric Point: 7.2544

>NTDB_id=237936 I3O_RS24010 WP_001345114.1 4513623..4515452(+) (recQ) [Escherichia coli O111 str. RM9322]
MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCLVVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKD
QVDQLQVNGVAAACLNSTQTREQQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGI
IYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRADVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESY
YQETGRAGRDGLPAEAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQEPCGNC
DICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDYGHDKLKVYGMGRDKSHEHWVSVIRQLIHLG
LVTQNIAQHSALQLTEAARPVLRGESSLQLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFND
ATLIEMAEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=237936 I3O_RS24010 WP_001345114.1 4513623..4515452(+) (recQ) [Escherichia coli O111 str. RM9322]
GTGGCGCAGGCGGAAGTGTTGAATCTGGAGTCCGGAGCTAAACAGGTTTTACAAGAAACCTTTGGCTACCAACAGTTTCG
CCCCGGCCAGGAAGAAATTATCGACACTGTGCTTTCCGGTCGCGATTGCCTGGTCGTCATGCCCACCGGTGGCGGAAAAT
CCCTTTGCTATCAAATTCCTGCCTTATTGCTAAACGGCCTTACCGTGGTTGTTTCACCGCTGATTTCGTTGATGAAAGAT
CAGGTGGATCAACTGCAAGTCAACGGCGTGGCGGCGGCGTGCCTTAACTCGACGCAAACCCGCGAACAGCAACTTGAAGT
GATGACAGGCTGCCGCACCGGGCAAATTCGTCTGCTTTATATCGCCCCGGAACGCCTGATGCTGGATAACTTTCTTGAGC
ATCTGGCGCACTGGAATCCGGTGTTATTAGCCGTTGATGAAGCGCACTGTATCTCCCAATGGGGCCACGATTTCCGCCCG
GAATATGCCGCGCTCGGTCAGTTGCGCCAGCGGTTCCCGACGCTGCCGTTTATGGCGCTGACCGCCACAGCCGACGACAC
CACGCGCCAGGATATCGTGCGCCTGCTGGGGCTGAACGATCCGCTGATTCAAATCAGCAGTTTTGACCGTCCGAATATTC
GCTACATGCTGATGGAGAAGTTCAAACCGCTCGATCAGTTGATGCGCTACGTGCAGGAACAGCGCGGTAAGTCCGGCATT
ATCTACTGCAACAGCCGGGCGAAAGTAGAAGACACCGCTGCGCGCCTGCAAAGCAAGGGTATTAGCGCGGCGGCCTATCA
TGCCGGGCTGGAAAATAATGTCCGCGCCGACGTGCAGGAGAAATTCCAGCGCGATGACCTGCAAATTGTGGTGGCGACGG
TGGCGTTCGGCATGGGCATCAATAAACCAAACGTTCGCTTCGTGGTCCACTTTGATATTCCGCGCAATATCGAATCCTAT
TATCAGGAAACCGGTCGCGCCGGGCGTGATGGCCTGCCCGCGGAAGCGATGCTGTTTTACGATCCGGCCGATATGGCGTG
GCTGCGCCGTTGTCTGGAAGAGAAGCCGCAGGGGCAGTTGCAGGATATCGAACGTCATAAACTCAATGCGATGGGCGCTT
TTGCCGAAGCGCAAACTTGCCGTCGTCTGGTACTGCTGAACTATTTTGGCGAAGGGCGTCAGGAGCCGTGCGGGAACTGC
GATATCTGCCTCGATCCGCCGAAACAGTACGACGGTTCAACCGATGCTCAGATTGCCCTTTCCACCATTGGTCGTGTGAA
TCAGCGTTTTGGTATGGGCTATGTGGTGGAAGTGATTCGCGGCGCTAATAACCAGCGTATCCGCGACTATGGTCATGACA
AACTAAAAGTCTACGGTATGGGCCGTGATAAAAGTCATGAACATTGGGTGAGCGTGATCCGCCAGCTGATTCACCTCGGT
CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCTGCGCGTCCGGTGCTGCGCGGCGAATCCTC
TTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA
AACTGTTCGCCAAATTACGCAAACTGCGTAAATCGATAGCCGATGAAAGTAATGTCCCGCCGTACGTGGTGTTTAACGAC
GCAACCTTGATTGAGATGGCTGAACAGATGCCGATCACCGCCAGCGAAATGCTCAGCGTTAACGGCGTTGGGATGCGCAA
GCTGGAACGCTTTGGCAAACCGTTTATGGCGCTGATTCGTGCGCATGTTGATGGCGATGACGAAGAGTAG

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(28-187)

RecQ zinc-binding (RecQ_Zn_bind)

(343-404)

Helicase conserved C-terminal domain (Helicase_C)

(223-330)

HRDC domain (HRDC)

(533-599)

RQC domain (RQC)

(406-514)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recQ Bacillus subtilis subsp. subtilis str. 168

40.203

97.209

0.391

Multiple sequence alignment