Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A8H28_RS22205 Genome accession   NZ_CP022213
Coordinates   1003571..1005076 (-) Length   501 a.a.
NCBI ID   WP_036049140.1    Uniprot ID   A0AAW7R6G5
Organism   Burkholderia gladioli pv. gladioli strain FDAARGOS_188     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 998571..1010076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8H28_RS22180 (A8H28_22030) - 999563..1000198 (-) 636 WP_036049137.1 LON peptidase substrate-binding domain-containing protein -
  A8H28_RS22185 (A8H28_22035) rapZ 1000231..1001124 (-) 894 WP_013699411.1 RNase adapter RapZ -
  A8H28_RS22190 (A8H28_22040) hprK 1001235..1002203 (-) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  A8H28_RS22195 (A8H28_22045) - 1002329..1002841 (-) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  A8H28_RS22200 (A8H28_22050) hpf 1003055..1003420 (-) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A8H28_RS22205 (A8H28_22055) rpoN/rpoN1 1003571..1005076 (-) 1506 WP_036049140.1 RNA polymerase factor sigma-54 Machinery gene
  A8H28_RS22210 (A8H28_22060) lptB 1005267..1006043 (-) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  A8H28_RS22215 (A8H28_22065) lptA 1006040..1006708 (-) 669 WP_036049142.1 lipopolysaccharide transport periplasmic protein LptA -
  A8H28_RS22220 (A8H28_22070) lptC 1006727..1007341 (-) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  A8H28_RS22225 (A8H28_22075) - 1007345..1007884 (-) 540 WP_036049144.1 HAD family hydrolase -
  A8H28_RS22230 (A8H28_22080) kdsD 1007884..1008867 (-) 984 WP_036049146.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55077.04 Da        Isoelectric Point: 4.9483

>NTDB_id=237593 A8H28_RS22205 WP_036049140.1 1003571..1005076(-) (rpoN/rpoN1) [Burkholderia gladioli pv. gladioli strain FDAARGOS_188]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEASGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=237593 A8H28_RS22205 WP_036049140.1 1003571..1005076(-) (rpoN/rpoN1) [Burkholderia gladioli pv. gladioli strain FDAARGOS_188]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CGAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAAGCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCATGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCACACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment