Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CEW83_RS20200 Genome accession   NZ_CP022187
Coordinates   4409688..4411142 (-) Length   484 a.a.
NCBI ID   WP_108950965.1    Uniprot ID   A0A2U8GW67
Organism   Parazoarcus communis strain TSPY31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4404688..4416142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW83_RS20175 (CEW83_20160) - 4405133..4405954 (-) 822 WP_234418929.1 substrate-binding periplasmic protein -
  CEW83_RS20180 (CEW83_20165) ilvA 4406163..4407683 (-) 1521 WP_108950961.1 threonine ammonia-lyase, biosynthetic -
  CEW83_RS20185 (CEW83_20170) hprK 4407797..4408759 (-) 963 WP_108950962.1 HPr(Ser) kinase/phosphatase -
  CEW83_RS20190 (CEW83_20175) ptsN 4408728..4409213 (-) 486 WP_108950963.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CEW83_RS20195 (CEW83_20180) hpf 4409347..4409670 (-) 324 WP_108950964.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CEW83_RS20200 (CEW83_20185) rpoN/rpoN1 4409688..4411142 (-) 1455 WP_108950965.1 RNA polymerase factor sigma-54 Machinery gene
  CEW83_RS20205 (CEW83_20190) lptB 4411167..4411889 (-) 723 WP_108950966.1 LPS export ABC transporter ATP-binding protein -
  CEW83_RS20210 (CEW83_20195) recX 4411876..4412325 (-) 450 WP_108950967.1 recombination regulator RecX -
  CEW83_RS20215 (CEW83_20200) recA 4412355..4413392 (-) 1038 WP_108950968.1 recombinase RecA Machinery gene
  CEW83_RS20220 (CEW83_20205) - 4413421..4413939 (-) 519 WP_108950969.1 CinA family protein -
  CEW83_RS20225 (CEW83_20210) - 4413944..4414435 (-) 492 WP_234418930.1 phosphatidylglycerophosphatase A -
  CEW83_RS20230 (CEW83_20215) thiL 4414428..4415387 (-) 960 WP_108950970.1 thiamine-phosphate kinase -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 54196.96 Da        Isoelectric Point: 4.6491

>NTDB_id=237476 CEW83_RS20200 WP_108950965.1 4409688..4411142(-) (rpoN/rpoN1) [Parazoarcus communis strain TSPY31]
MKPTLQLKLSQHLTLTPQLQQSIKLLQLSTLELNQEIERFLLENPMLEREDGDGSDFAPSAVGNDAQPATETPRETSAET
NQEAEPRNENESADFDEGMDWSGTGSGASSKSDDDEDSDFQEFQAAGTSLRDYLDQQVALSPLTDRDRMLVRFIIEALDS
DGYLHQELEDLLELLPPELDYDLDDLAIALRHVQQLEPAGIGARTPQECLGLQLRAMPAGSCRDLALVIVEQHLELLAER
NFAKLKRLTGCDDDALRDAQALVCRLDPHPGSQHSNEETRYVLPDVVVRKIRNRWTVSLNAEAMPRLRINQLYASLLQQN
RGQGGGLTGQLQEARWLIKNVQQRFDTILRVSQAIVDQQRQFFDHGEVAMRPLTLREIADQLELHESTVSRVTTQKYMAT
PRGVFELKYFFGSHVATDTGGAASSTAIRALIRQLVDAEDRKKPLSDAKIADLLGQQGIVVARRTIAKYRESLNIPPVSL
RKTL

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=237476 CEW83_RS20200 WP_108950965.1 4409688..4411142(-) (rpoN/rpoN1) [Parazoarcus communis strain TSPY31]
ATGAAACCAACCCTTCAGCTCAAGCTCTCGCAGCACCTGACGCTCACCCCGCAGCTGCAGCAGTCCATCAAACTGCTGCA
GCTCTCTACGCTCGAGCTCAACCAGGAAATCGAACGCTTTCTGCTGGAAAACCCGATGCTCGAGCGTGAGGACGGCGACG
GCAGCGACTTCGCACCGTCCGCGGTCGGCAACGATGCGCAGCCCGCAACCGAGACCCCGCGCGAGACCAGCGCCGAGACC
AACCAGGAAGCCGAGCCCCGCAACGAGAACGAATCGGCCGACTTTGACGAAGGCATGGACTGGTCGGGCACCGGCAGCGG
CGCCAGCAGCAAGAGCGACGACGACGAGGACAGCGACTTCCAGGAATTCCAGGCCGCAGGCACCTCGCTGCGCGACTATC
TCGATCAGCAGGTTGCCCTGTCCCCGCTGACGGACCGTGACCGCATGCTGGTGCGCTTCATCATCGAAGCGCTCGATAGC
GACGGCTACCTGCACCAGGAGCTCGAGGACCTGCTCGAACTGCTCCCGCCCGAACTCGACTACGACCTCGACGACCTCGC
GATTGCCCTGCGGCACGTGCAGCAGCTCGAACCGGCGGGCATTGGCGCACGCACCCCCCAGGAGTGCCTCGGCCTGCAGC
TGCGCGCCATGCCGGCAGGCAGCTGCCGCGACCTGGCGCTGGTCATCGTCGAACAGCACCTCGAGCTGCTGGCCGAACGC
AACTTTGCGAAGCTCAAGCGCCTCACCGGGTGCGACGACGACGCCCTGCGCGATGCCCAGGCGCTGGTGTGCCGGCTCGA
CCCGCATCCGGGGTCGCAGCATTCCAACGAAGAAACCCGCTACGTCCTGCCCGATGTCGTGGTGCGCAAGATCCGCAACC
GGTGGACGGTGTCGCTCAACGCCGAAGCCATGCCGCGCCTGCGCATCAACCAGCTCTACGCCAGCCTGCTGCAGCAGAAC
CGGGGCCAGGGCGGCGGTCTGACCGGCCAGCTGCAGGAAGCACGCTGGCTGATCAAGAACGTACAACAGCGCTTTGATAC
CATTTTGCGTGTATCTCAGGCCATCGTTGACCAGCAGCGACAGTTCTTCGATCATGGCGAGGTGGCAATGCGTCCATTGA
CCCTCAGGGAGATCGCGGACCAGCTCGAGCTGCATGAATCAACCGTATCGCGCGTCACCACTCAGAAGTACATGGCGACG
CCGCGCGGCGTATTCGAACTCAAGTATTTTTTTGGCAGTCACGTTGCCACCGACACCGGTGGTGCGGCTTCTTCCACAGC
GATTCGTGCCTTGATTCGCCAGCTGGTGGACGCCGAAGACAGAAAGAAACCCCTGTCCGACGCAAAGATTGCCGATCTGT
TGGGCCAGCAGGGTATCGTGGTCGCGCGGCGCACAATCGCCAAATACCGCGAATCACTCAATATCCCGCCGGTCAGTCTG
CGTAAAACGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-482)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(120-313)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GW67

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

55.578

100

0.576

Multiple sequence alignment