Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CE462_RS21350 Genome accession   NZ_CP022186
Coordinates   4346675..4348114 (-) Length   479 a.a.
NCBI ID   WP_005314410.1    Uniprot ID   A4SHX7
Organism   Aeromonas salmonicida strain S68     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4341675..4353114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CE462_RS21320 (CE462_21320) dagF 4341948..4342688 (+) 741 WP_005314402.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  CE462_RS21325 (CE462_21325) - 4342792..4344696 (+) 1905 WP_042468040.1 BglG family transcription antiterminator -
  CE462_RS21330 (CE462_21330) npr 4344715..4344987 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  CE462_RS21335 (CE462_21335) rapZ 4345008..4345874 (-) 867 WP_005314405.1 RNase adapter RapZ -
  CE462_RS21340 (CE462_21340) ptsN 4345917..4346363 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CE462_RS21345 (CE462_21345) hpf 4346366..4346653 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  CE462_RS21350 (CE462_21350) rpoN/rpoN1 4346675..4348114 (-) 1440 WP_005314410.1 RNA polymerase factor sigma-54 Machinery gene
  CE462_RS21355 (CE462_21355) lptB 4348180..4348905 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  CE462_RS21360 (CE462_21360) lptA 4348909..4349436 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  CE462_RS21365 (CE462_21365) lptC 4349423..4349983 (-) 561 WP_042468042.1 LPS export ABC transporter periplasmic protein LptC -
  CE462_RS21370 (CE462_21370) kdsC 4349980..4350534 (-) 555 WP_042468044.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CE462_RS21375 (CE462_21375) - 4350534..4351529 (-) 996 WP_011898279.1 KpsF/GutQ family sugar-phosphate isomerase -
  CE462_RS21380 (CE462_21380) mlaF 4351737..4352537 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53962.92 Da        Isoelectric Point: 4.3718

>NTDB_id=237450 CE462_RS21350 WP_005314410.1 4346675..4348114(-) (rpoN/rpoN1) [Aeromonas salmonicida strain S68]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGRECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=237450 CE462_RS21350 WP_005314410.1 4346675..4348114(-) (rpoN/rpoN1) [Aeromonas salmonicida strain S68]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGACG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAAGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCCGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCAGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTAATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGGGCAACAAATGGGTCGTTGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAAGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.58

100

0.484

Multiple sequence alignment