Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CEJ46_RS12650 Genome accession   NZ_CP022101
Coordinates   2734310..2735320 (-) Length   336 a.a.
NCBI ID   WP_088721345.1    Uniprot ID   -
Organism   Vibrio anguillarum strain JLL237     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2729310..2740320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEJ46_RS12625 (CEJ46_12625) aroK 2730177..2730698 (-) 522 WP_010317691.1 shikimate kinase AroK -
  CEJ46_RS12630 (CEJ46_12630) pilQ 2730885..2732600 (-) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  CEJ46_RS12635 (CEJ46_12635) pilP 2732624..2733145 (-) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  CEJ46_RS12640 (CEJ46_12640) pilO 2733132..2733725 (-) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  CEJ46_RS12645 (CEJ46_12645) pilN 2733718..2734320 (-) 603 WP_064625964.1 PilN domain-containing protein Machinery gene
  CEJ46_RS12650 (CEJ46_12650) pilM 2734310..2735320 (-) 1011 WP_088721345.1 type IV pilus assembly protein PilM Machinery gene
  CEJ46_RS12655 (CEJ46_12655) - 2735470..2737968 (+) 2499 WP_088721346.1 penicillin-binding protein 1A -
  CEJ46_RS12660 (CEJ46_12660) oxyR 2738005..2738895 (-) 891 WP_026027425.1 DNA-binding transcriptional regulator OxyR -
  CEJ46_RS12665 (CEJ46_12665) - 2739042..2739770 (+) 729 WP_013855882.1 redoxin family protein -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37454.99 Da        Isoelectric Point: 7.5395

>NTDB_id=236791 CEJ46_RS12650 WP_088721345.1 2734310..2735320(-) (pilM) [Vibrio anguillarum strain JLL237]
MGKSLVTGIDIGHHSIKAVVLKLAGDSYALVGYKELFITEDIFSDNHTLDYQKIVKKLKELRKGLPLFSRKVAISVPDNA
VISKVLQIDSDLQAREKEFAIYQAFSHQSPFPIEELSLDFVKVMEKSVSRSSTVSYQVYATRKDVVESRALAAQKAGFLP
IAVDVQIHSLLNIWQLASRAQHRSDWMLMDVGHTQTALCIDFTDKAPFHKEIPLGCQWLSQDSAMSGTNAELADKNERFV
HELIERLQRQLQLFTSVNGAGSLAGIWLSGGGADTPMLAQEISRRLKLECEILDPFLLFNNKLAKRQRDKQSGQSFSTAA
GLALRGIEWLESSHVA

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=236791 CEJ46_RS12650 WP_088721345.1 2734310..2735320(-) (pilM) [Vibrio anguillarum strain JLL237]
ATGGGTAAATCATTGGTTACGGGTATAGATATTGGCCACCATAGTATTAAAGCGGTGGTGTTAAAGCTAGCGGGTGATTC
ATATGCACTCGTCGGTTATAAAGAATTATTTATTACTGAGGACATATTCTCCGATAACCACACGCTGGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGGGTTTACCACTATTTAGTCGCAAAGTCGCCATATCGGTACCGGACAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGTGACCTGCAGGCGCGTGAAAAAGAGTTTGCTATTTATCAGGCATTTTCTCA
TCAGTCACCGTTCCCGATAGAAGAACTGAGTCTCGATTTCGTCAAAGTGATGGAAAAAAGCGTAAGTCGCAGTAGCACCG
TAAGTTATCAAGTTTACGCCACTCGTAAAGACGTCGTGGAAAGCCGTGCGTTAGCGGCTCAAAAAGCGGGCTTTTTACCG
ATCGCGGTTGATGTGCAAATACACAGTTTGTTAAACATTTGGCAGTTGGCGAGCAGGGCACAACATCGCAGCGATTGGAT
GCTCATGGATGTGGGCCATACCCAAACGGCGCTCTGTATTGATTTTACTGATAAAGCCCCTTTTCATAAAGAGATCCCTC
TCGGTTGTCAGTGGTTGTCCCAAGACAGCGCGATGTCGGGCACCAATGCCGAATTAGCCGATAAAAACGAGCGCTTTGTG
CATGAGTTGATCGAGCGACTTCAACGTCAGTTGCAGCTTTTCACCTCCGTCAATGGCGCGGGCAGTTTAGCAGGCATTTG
GCTCTCAGGTGGTGGCGCAGATACTCCGATGTTGGCGCAAGAGATCTCACGGCGTCTCAAGCTTGAGTGCGAGATTCTCG
ATCCCTTCCTTCTGTTTAACAATAAGTTAGCGAAACGCCAGCGAGATAAGCAGTCTGGGCAGAGTTTTAGCACCGCAGCA
GGCCTTGCACTGCGTGGTATCGAGTGGTTGGAGTCTTCTCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

67.456

100

0.679
  pilM Vibrio campbellii strain DS40M4

52.976

100

0.53

Multiple sequence alignment