Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CYFUS_RS09115 Genome accession   NZ_CP022098
Coordinates   2169866..2171227 (-) Length   453 a.a.
NCBI ID   WP_095984867.1    Uniprot ID   A0A250IYL9
Organism   Cystobacter fuscus strain DSM 52655     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2164866..2176227
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYFUS_RS09100 (CYFUS_001804) - 2164949..2166118 (+) 1170 WP_095984864.1 efflux RND transporter periplasmic adaptor subunit -
  CYFUS_RS09105 (CYFUS_001805) - 2166140..2169295 (+) 3156 WP_095984865.1 efflux RND transporter permease subunit -
  CYFUS_RS09110 (CYFUS_001806) - 2169543..2169764 (+) 222 WP_095984866.1 hypothetical protein -
  CYFUS_RS09115 (CYFUS_001807) radA 2169866..2171227 (-) 1362 WP_095984867.1 DNA repair protein RadA Machinery gene
  CYFUS_RS09120 (CYFUS_001808) - 2171415..2171684 (+) 270 WP_095984868.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  CYFUS_RS09125 (CYFUS_001809) bioB 2171831..2172862 (+) 1032 WP_095984869.1 biotin synthase BioB -
  CYFUS_RS09130 (CYFUS_001810) bioF 2172859..2174031 (+) 1173 WP_095984870.1 8-amino-7-oxononanoate synthase -
  CYFUS_RS09135 (CYFUS_001811) bioD 2174043..2174756 (+) 714 WP_095984871.1 dethiobiotin synthase -
  CYFUS_RS09140 (CYFUS_001812) - 2174860..2175138 (+) 279 WP_095984872.1 hypothetical protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48025.15 Da        Isoelectric Point: 6.7594

>NTDB_id=236693 CYFUS_RS09115 WP_095984867.1 2169866..2171227(-) (radA) [Cystobacter fuscus strain DSM 52655]
MAKAKTHYACQACGYQSAKWLGKCPDCGAWSSLVEETETKVDEKRPAWGASGGASKPVRLREVSGEQEQRRHTGIAEFDR
VLGGGVVDGSLVLLGGDPGIGKSTLLLAALDRLGHAGPVLYVSGEESLRQTKMRAERLRVEGEAIHLFAETDADRVLSVA
ESLKPRALVVDSIQTMYLPELGSAPGSISQVREVAGRFMAYAKRSGVPTFLVGHVTKEGSIAGPRVLEHMVDTVLYFEGE
RGHPFRILRAHKNRFGSTNEIGVFEMKGAGLVEVADPSALFLAERPAGKAGSVVTCTLNGTRPLLVEVQALVAPTGYGTA
RRTAIGVDGNRVALLAAVLEKKEDIPLVGCDLFVNVAGGMQLSEPACDLAVCAALVSSLQNRPLEAHTLVLGEVGLAGEV
RAVGQVEPRLAEAAKMGFQRAVIPKGSARRLEDSKLEVVGVETLSEALGAMFD

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=236693 CYFUS_RS09115 WP_095984867.1 2169866..2171227(-) (radA) [Cystobacter fuscus strain DSM 52655]
ATGGCGAAGGCGAAGACGCATTACGCGTGTCAGGCGTGCGGGTACCAGTCGGCGAAGTGGTTGGGCAAATGCCCTGACTG
CGGCGCGTGGAGCTCGCTGGTGGAGGAGACCGAGACGAAGGTGGACGAGAAGCGCCCGGCCTGGGGCGCCTCGGGCGGAG
CCTCCAAGCCGGTGCGGCTGCGCGAGGTGAGCGGCGAGCAGGAGCAGCGCCGGCACACGGGCATCGCCGAGTTCGACCGG
GTCCTCGGTGGCGGAGTGGTGGACGGCTCTCTGGTGCTGCTCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGCT
CGCCGCGTTGGATCGGCTGGGCCACGCGGGCCCGGTGCTCTACGTGTCGGGCGAGGAGTCGCTGCGGCAGACGAAGATGC
GCGCCGAGCGCCTGCGGGTGGAGGGCGAGGCCATCCACCTCTTCGCGGAGACGGACGCGGACCGGGTGCTCTCGGTGGCC
GAGTCGCTCAAGCCCCGGGCGCTGGTGGTGGACTCCATCCAGACCATGTACCTGCCGGAGCTGGGCAGCGCGCCGGGGAG
CATCTCCCAGGTGCGCGAGGTGGCCGGGCGCTTCATGGCCTACGCCAAGCGCAGCGGCGTGCCCACCTTCCTCGTGGGCC
ACGTGACGAAGGAGGGCTCCATCGCGGGCCCGCGCGTGCTCGAGCACATGGTGGACACCGTCCTCTACTTCGAGGGCGAG
CGCGGCCACCCCTTCCGGATTCTGCGGGCGCACAAGAACCGCTTCGGCTCGACGAACGAGATTGGCGTCTTCGAGATGAA
GGGCGCGGGGCTGGTGGAGGTGGCCGACCCCTCGGCGCTCTTCCTCGCCGAGCGCCCGGCGGGCAAGGCGGGCAGCGTGG
TGACGTGCACGCTCAATGGCACGCGCCCCCTGCTGGTGGAGGTGCAGGCGCTGGTGGCGCCCACCGGCTACGGCACCGCG
CGGCGCACGGCCATCGGCGTGGATGGCAACCGCGTGGCCCTGCTCGCCGCGGTGTTGGAGAAGAAGGAGGACATTCCACT
GGTGGGCTGCGACCTCTTCGTCAACGTGGCCGGCGGCATGCAGTTGTCCGAGCCGGCATGTGACCTGGCGGTGTGCGCGG
CCCTGGTGAGCAGCCTGCAGAACCGGCCACTGGAGGCGCATACCCTGGTGCTCGGCGAGGTGGGACTCGCGGGCGAGGTG
CGCGCGGTGGGCCAGGTGGAGCCGCGACTCGCCGAGGCGGCGAAGATGGGCTTCCAGCGCGCCGTCATTCCCAAGGGCAG
CGCCCGGCGGCTCGAGGACTCCAAGCTGGAGGTGGTGGGCGTGGAGACGCTGTCCGAGGCGCTCGGCGCCATGTTCGACT
GA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(80-219)

Subunit ChlI of Mg-chelatase (ChlI)

(352-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A250IYL9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

54.204

99.779

0.541
  radA Streptococcus mitis NCTC 12261

52.98

100

0.53
  radA Streptococcus pneumoniae Rx1

52.98

100

0.53
  radA Streptococcus pneumoniae D39

52.98

100

0.53
  radA Streptococcus pneumoniae R6

52.98

100

0.53
  radA Streptococcus pneumoniae TIGR4

52.98

100

0.53
  radA Streptococcus mitis SK321

52.759

100

0.528

Multiple sequence alignment