Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   BCE33L_RS21295 Genome accession   NC_006274
Coordinates   4103538..4104581 (-) Length   347 a.a.
NCBI ID   WP_001013208.1    Uniprot ID   Q634U2
Organism   Bacillus cereus E33L     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4098538..4109581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCE33L_RS21250 (BCE33L3986) - 4098965..4099165 (-) 201 WP_000106081.1 YqzE family protein -
  BCE33L_RS21255 (BCE33L3987) aroK 4099204..4099701 (-) 498 WP_000836637.1 shikimate kinase AroK -
  BCE33L_RS21260 (BCE33L3988) - 4099822..4100472 (-) 651 WP_000183877.1 2OG-Fe(II) oxygenase -
  BCE33L_RS21265 (BCE33L3989) comGG 4100648..4101019 (-) 372 WP_001231187.1 competence type IV pilus minor pilin ComGG -
  BCE33L_RS21270 (BCE33L3990) comGF 4101016..4101486 (-) 471 WP_000923093.1 competence type IV pilus minor pilin ComGF -
  BCE33L_RS21275 (BCE33L3991) comGE 4101456..4101758 (-) 303 WP_000829458.1 competence type IV pilus minor pilin ComGE -
  BCE33L_RS21280 (BCE33L3992) comGD 4101751..4102206 (-) 456 WP_000810395.1 comG operon protein ComGD -
  BCE33L_RS21285 (BCE33L3993) comGC 4102203..4102502 (-) 300 WP_001178682.1 comG operon protein ComGC -
  BCE33L_RS21290 (BCE33L3994) comGB 4102514..4103545 (-) 1032 WP_000471908.1 comG operon protein ComGB -
  BCE33L_RS21295 (BCE33L3995) comGA 4103538..4104581 (-) 1044 WP_001013208.1 competence protein ComGA Machinery gene
  BCE33L_RS21300 (BCE33L3996) - 4104786..4105481 (+) 696 WP_000434116.1 helix-turn-helix transcriptional regulator -
  BCE33L_RS21305 (BCE33L3997) - 4105607..4105849 (+) 243 WP_000440713.1 DUF2626 domain-containing protein -
  BCE33L_RS21310 (BCE33L3998) - 4105957..4107351 (+) 1395 WP_001094329.1 L-cystine transporter -
  BCE33L_RS21315 (BCE33L3999) - 4107567..4107782 (-) 216 WP_001008322.1 DUF3912 family protein -
  BCE33L_RS21320 (BCE33L4000) - 4108035..4108346 (+) 312 WP_001093243.1 hypothetical protein -
  BCE33L_RS21325 (BCE33L4001) - 4108383..4108871 (-) 489 WP_000764246.1 hypothetical protein -
  BCE33L_RS21330 (BCE33L4002) - 4108951..4109325 (-) 375 WP_001012349.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39287.78 Da        Isoelectric Point: 9.2324

>NTDB_id=23641 BCE33L_RS21295 WP_001013208.1 4103538..4104581(-) (comGA) [Bacillus cereus E33L]
MNGIESFANTILKEACRVQASDLHIVPRQKDVVIQLRIGKDLMTKQCIEKGFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRSDDLLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGTILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=23641 BCE33L_RS21295 WP_001013208.1 4103538..4104581(-) (comGA) [Bacillus cereus E33L]
ATGAATGGAATTGAAAGCTTTGCGAATACGATTTTGAAAGAAGCGTGTAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTAATTCAACTGCGTATAGGAAAAGATTTAATGACGAAACAATGCATTGAAAAGGGGTTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAGGAAGTGTATTTACGTCTTTCCACGCTTCCAACTGTATATCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
ATTATTCCCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGCCGCATCGTTACATTGGAGGATCCAGTTGAAAAAAGAAGTGACGATTTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACGAATGATGCGAGAGGGACGATACTCAGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q634U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.349

100

0.573
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366

Multiple sequence alignment