Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   BCE33L_RS20045 Genome accession   NC_006274
Coordinates   3898804..3899238 (-) Length   144 a.a.
NCBI ID   WP_000811492.1    Uniprot ID   A0A0G8EA73
Organism   Bacillus cereus E33L     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3893804..3904238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCE33L_RS20025 (BCE33L3759) - 3894575..3895132 (+) 558 WP_000062070.1 PadR family transcriptional regulator -
  BCE33L_RS20035 (BCE33L3760) metE 3896053..3898341 (+) 2289 WP_001007638.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  BCE33L_RS20040 (BCE33L3761) comJ 3898378..3898767 (-) 390 WP_000424034.1 competence protein ComJ -
  BCE33L_RS20045 (BCE33L3762) nucA/comI 3898804..3899238 (-) 435 WP_000811492.1 DNA-entry nuclease Machinery gene
  BCE33L_RS20050 (BCE33L3763) - 3899475..3900224 (+) 750 WP_000388474.1 DUF3967 domain-containing protein -
  BCE33L_RS20055 (BCE33L3764) kinB 3900278..3901552 (-) 1275 WP_000420382.1 sporulation sensor histidine kinase KinB -
  BCE33L_RS20060 (BCE33L3765) - 3901862..3902062 (+) 201 WP_000929284.1 hypothetical protein -
  BCE33L_RS20065 (BCE33L3766) - 3902186..3903349 (+) 1164 WP_000434588.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16012.35 Da        Isoelectric Point: 8.4331

>NTDB_id=23638 BCE33L_RS20045 WP_000811492.1 3898804..3899238(-) (nucA/comI) [Bacillus cereus E33L]
MKQLKGIIISIIAILSILVAVYEVLVPEETSTKKTNAYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=23638 BCE33L_RS20045 WP_000811492.1 3898804..3899238(-) (nucA/comI) [Bacillus cereus E33L]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTTTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTACAAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGAGAGATATCCAGAAACAGGGA
AACATATTACGGATGCGATAAAGGAAGGACATTCAGAAGTTTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAG
TTATCGTTAGCCCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCAGCAGATAATCGAGGAGCAGGGTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8EA73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

70.796

78.472

0.556

Multiple sequence alignment