Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CEG11_RS08940 Genome accession   NZ_CP021976
Coordinates   1795827..1796261 (+) Length   144 a.a.
NCBI ID   WP_003154085.1    Uniprot ID   -
Organism   Bacillus velezensis strain T20E-257     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1790827..1801261
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEG11_RS08935 (CEG11_08935) - 1794327..1795538 (-) 1212 WP_003154086.1 cytochrome P450 family protein -
  CEG11_RS08940 (CEG11_08940) nucA/comI 1795827..1796261 (+) 435 WP_003154085.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CEG11_RS08945 (CEG11_08945) - 1796321..1797076 (+) 756 WP_003154084.1 YoaK family protein -
  CEG11_RS08950 (CEG11_08950) - 1797110..1797472 (-) 363 WP_003154082.1 hypothetical protein -
  CEG11_RS08955 (CEG11_08955) - 1797664..1798992 (-) 1329 WP_003154079.1 S8 family peptidase -
  CEG11_RS08960 (CEG11_08960) - 1799172..1799405 (+) 234 WP_031306556.1 hypothetical protein -
  CEG11_RS08965 (CEG11_08965) - 1799661..1800368 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  CEG11_RS08970 (CEG11_08970) - 1800428..1800880 (+) 453 WP_003154075.1 OsmC family protein -
  CEG11_RS08975 (CEG11_08975) - 1800894..1801247 (-) 354 WP_003154073.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15509.42 Da        Isoelectric Point: 6.8606

>NTDB_id=235497 CEG11_RS08940 WP_003154085.1 1795827..1796261(+) (nucA/comI) [Bacillus velezensis strain T20E-257]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=235497 CEG11_RS08940 WP_003154085.1 1795827..1796261(+) (nucA/comI) [Bacillus velezensis strain T20E-257]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment