Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CD200_RS13260 Genome accession   NZ_CP021970
Coordinates   2540114..2541052 (-) Length   312 a.a.
NCBI ID   WP_061565980.1    Uniprot ID   A0A8B5Y947
Organism   Bacillus licheniformis strain CBA7132     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535114..2546052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD200_RS13215 (CD200_13270) tapA 2535614..2536342 (-) 729 WP_003183451.1 amyloid fiber anchoring/assembly protein TapA -
  CD200_RS13220 (CD200_13275) - 2536652..2536939 (+) 288 WP_223307118.1 YqzG/YhdC family protein -
  CD200_RS13225 (CD200_13280) - 2536963..2537145 (-) 183 WP_003183456.1 YqzE family protein -
  CD200_RS13230 (CD200_13285) comGG 2537234..2537599 (-) 366 WP_003183459.1 competence type IV pilus minor pilin ComGG -
  CD200_RS13235 (CD200_13290) comGF 2537612..2538100 (-) 489 WP_011201694.1 competence type IV pilus minor pilin ComGF -
  CD200_RS13240 (CD200_13295) comGE 2538009..2538356 (-) 348 WP_009327907.1 competence type IV pilus minor pilin ComGE -
  CD200_RS13245 (CD200_13300) comGD 2538340..2538777 (-) 438 WP_009327905.1 competence type IV pilus minor pilin ComGD -
  CD200_RS13250 (CD200_13305) comGC 2538783..2539076 (-) 294 WP_003183466.1 competence type IV pilus major pilin ComGC Machinery gene
  CD200_RS13255 (CD200_13310) comGB 2539090..2540124 (-) 1035 WP_011198115.1 competence type IV pilus assembly protein ComGB Machinery gene
  CD200_RS13260 (CD200_13315) comGA 2540114..2541052 (-) 939 WP_061565980.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  CD200_RS13265 (CD200_13320) - 2541209..2542048 (-) 840 WP_003183480.1 STAS domain-containing protein -
  CD200_RS13275 (CD200_13330) - 2542290..2542667 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  CD200_RS13280 (CD200_13335) - 2542876..2543121 (+) 246 WP_003183483.1 DUF2626 domain-containing protein -
  CD200_RS13285 (CD200_13340) - 2543159..2543797 (-) 639 WP_003183485.1 MBL fold metallo-hydrolase -
  CD200_RS13290 (CD200_13345) - 2543953..2544126 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  CD200_RS13295 (CD200_13350) - 2544187..2544501 (-) 315 WP_003183487.1 MTH1187 family thiamine-binding protein -
  CD200_RS13300 (CD200_13355) - 2544517..2545626 (-) 1110 WP_003183488.1 hypothetical protein -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35224.00 Da        Isoelectric Point: 9.1258

>NTDB_id=235354 CD200_RS13260 WP_061565980.1 2540114..2541052(-) (comGA) [Bacillus licheniformis strain CBA7132]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDGLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINREPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLMLFTGPTGSGKTTTLYSLIDY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVSAQRLVDIVCPFCG
DSCTLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRCGGIKTDTLKMLIRKGIALGYLPSKTYERWIGHED

Nucleotide


Download         Length: 939 bp        

>NTDB_id=235354 CD200_RS13260 WP_061565980.1 2540114..2541052(-) (comGA) [Bacillus licheniformis strain CBA7132]
TTGTACACGATCGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAAGAGGCGGTGGTACGCTTCAGGATTGATGACGAACTGTTTCAAAAAGACGGATTAACGAGAAATGAGTGCT
CTAGACTCATTTCTCACTTTAAATTTCTTTCTTCGATGGACATTGGCGAGAGACGGCTGCCGCAAAGCGGGGCTTTAACT
CTTTATATCAACCGTGAGCCCGTTCATTTAAGGATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTCCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATGCTTTTCACCGGCCCGACAGGCTCGGGAAAAACGACAACACTCTATTCTTTAATTGACTAC
GCCAAGCGGCATTTTAAGCGAAATATCATTACGTTGGAAGACCCGGTCGAATCAAGAAGCGAAAACATTTTGCAAGTACA
AGTGAACGAAAAAGCGGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTGCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGCGCCCAGCGTCTTGTCGATATCGTCTGTCCGTTTTGCGGC
GACAGCTGCACTTTGTATTGCAGATTGTCGAGACCAGTTAGAAGAGCAAGCGTCTTTGAACTTTTGTACGGAAAAAGCCT
TAATCTCTGCATCGAAGAAGCAAAAGGCAGATGCGGTGGCATCAAAACAGACACCTTAAAAATGCTGATCCGCAAGGGAA
TAGCACTCGGATATCTGCCGTCGAAAACTTATGAACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A8B5Y947

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.757

100

0.667

Multiple sequence alignment