Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C6W78_RS01255 Genome accession   NZ_CP027461
Coordinates   242470..242910 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 95-3322     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 237470..247910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W78_RS01235 coaE 237960..238580 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  C6W78_RS26565 - 238605..238649 (+) 45 WP_120795372.1 protein YacM -
  C6W78_RS01240 guaC 238805..239848 (+) 1044 WP_001217338.1 GMP reductase -
  C6W78_RS01245 hofC 239883..241085 (-) 1203 WP_042022836.1 protein transport protein HofC -
  C6W78_RS01250 pilB 241075..242460 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  C6W78_RS01255 pilA 242470..242910 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  C6W78_RS01265 nadC 243114..244007 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C6W78_RS01270 ampD 244095..244646 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C6W78_RS01275 ampE 244643..245497 (+) 855 WP_021540728.1 beta-lactamase regulator AmpE -
  C6W78_RS01280 aroP 245540..246910 (-) 1371 WP_042022833.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=234612 C6W78_RS01255 WP_000360914.1 242470..242910(-) (pilA) [Escherichia coli strain 95-3322]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=234612 C6W78_RS01255 WP_000360914.1 242470..242910(-) (pilA) [Escherichia coli strain 95-3322]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment