Detailed information    

insolico Bioinformatically predicted

Overview


Name   nucA/comI   Type   Machinery gene
Locus tag   S100333_RS02000 Genome accession   NZ_CP021892
Coordinates   370004..370453 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. subtilis strain SRCM100333     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context


Location: 365004..375453
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S100333_RS22210 - 365335..365532 (+) 198 WP_121509395.1 hypothetical protein -
  S100333_RS01965 (S100333_00397) yckC 365552..366007 (+) 456 WP_014478793.1 RDD family protein -
  S100333_RS01970 (S100333_00398) yckD 366089..366421 (+) 333 WP_014478794.1 YckD family protein -
  S100333_RS01975 (S100333_00399) - 366549..366971 (+) 423 WP_014478795.1 MarR family transcriptional regulator -
  S100333_RS01980 (S100333_00400) - 366959..367279 (+) 321 WP_014478796.1 DUF3147 family protein -
  S100333_RS01985 (S100333_00401) - 367298..368042 (+) 745 Protein_344 AAA family ATPase -
  S100333_RS01990 (S100333_00404) bglC 368115..369542 (+) 1428 Protein_345 6-phospho-beta-glucosidase -
  S100333_RS01995 (S100333_00406) nin/comJ 369579..369977 (-) 399 WP_014478800.1 DNA-entry nuclease inhibitor Regulator
  S100333_RS02000 (S100333_00407) nucA/comI 370004..370453 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  S100333_RS02005 tlpC 370621..372342 (-) 1722 Protein_348 methyl-accepting chemotaxis protein TlpC -
  S100333_RS02010 (S100333_00410) hxlB 372452..373009 (-) 558 WP_014478803.1 6-phospho-3-hexuloisomerase -
  S100333_RS02015 (S100333_00411) hxlA 373015..373647 (-) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  S100333_RS02020 (S100333_00412) hxlR 373875..374237 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence


Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=234504 S100333_RS02000 WP_014475770.1 370004..370453(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SRCM100333]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=234504 S100333_RS02000 WP_014475770.1 370004..370453(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SRCM100333]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987


Multiple sequence alignment