Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6W79_RS13665 Genome accession   NZ_CP027457
Coordinates   2736972..2737952 (+) Length   326 a.a.
NCBI ID   WP_001613794.1    Uniprot ID   -
Organism   Escherichia coli strain 88-3493     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731972..2742952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6W79_RS13635 - 2732350..2733336 (-) 987 WP_001613796.1 TRAP transporter substrate-binding protein -
  C6W79_RS13640 hemW 2733649..2734785 (-) 1137 WP_000239917.1 radical SAM family heme chaperone HemW -
  C6W79_RS13645 rdgB 2734778..2735371 (-) 594 WP_001174751.1 XTP/dITP diphosphatase -
  C6W79_RS13650 yggU 2735379..2735669 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  C6W79_RS13655 yggT 2735666..2736232 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  C6W79_RS13660 yggS 2736250..2736954 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  C6W79_RS13665 pilT 2736972..2737952 (+) 981 WP_001613794.1 type IV pilus twitching motility protein PilT Machinery gene
  C6W79_RS13670 ruvX 2738115..2738531 (-) 417 WP_000017103.1 Holliday junction resolvase RuvX -
  C6W79_RS13675 yqgE 2738531..2739094 (-) 564 WP_106888500.1 YqgE/AlgH family protein -
  C6W79_RS13680 gshB 2739203..2740153 (-) 951 WP_000593273.1 glutathione synthase -
  C6W79_RS13685 rsmE 2740166..2740897 (-) 732 WP_001222509.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  C6W79_RS13690 endA 2740977..2741684 (-) 708 WP_001613793.1 deoxyribonuclease I -
  C6W79_RS13695 yggI 2741779..2742276 (-) 498 WP_001300769.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36019.31 Da        Isoelectric Point: 5.9945

>NTDB_id=234504 C6W79_RS13665 WP_001613794.1 2736972..2737952(+) (pilT) [Escherichia coli strain 88-3493]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLTKNQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGTPQILPELLKSENGLILVTGATGCGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=234504 C6W79_RS13665 WP_001613794.1 2736972..2737952(+) (pilT) [Escherichia coli strain 88-3493]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGACGAAAAACCAGCGATTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CACACCACAGATATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGTGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTGCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(61-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

46.789

100

0.469
  pilT Acinetobacter baylyi ADP1

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.063

97.239

0.39
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

37.346

99.387

0.371

Multiple sequence alignment